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AKAP13 Polyclonal antibody

AKAP13 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 27947-1-AP

Print datasheet

Synonyms

A kinase anchor protein 13, AKAP 13, AKAP Lbc, AKAP13, ARHGEF13, BRX, c lbc, HA 3, Ht31, LBC, LBC oncogene, Lymphoid blast crisis oncogene, p47, PROTO LB, PROTO LBC

验证数据展示

  • WB analysis using 27947-1-AP

    WB analysis using 27947-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 27947-1-AP (AKAP13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 27947-1-AP

    IHC staining of human breast cancer using 27947-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27947-1-AP (AKAP13 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AKAP13 antibody included
  • IHC staining of human breast cancer using 27947-1-AP

    IHC staining of human breast cancer using 27947-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27947-1-AP (AKAP13 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with AKAP13 antibody included

经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy AKAP13 Ready-To-Use IHC Kit. AKAP13 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27947-1-AP targets AKAP13 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human
Immunogen AKAP13 fusion protein Ag27486 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name A kinase (PRKA) anchor protein 13
Synonyms A kinase anchor protein 13, AKAP 13, AKAP Lbc, AKAP13, ARHGEF13, BRX, c lbc, HA 3, Ht31, LBC, LBC oncogene, Lymphoid blast crisis oncogene, p47, PROTO LB, PROTO LBC
Calculated Molecular Weight 308 kDa
GenBank Accession NumberNM_001270546
Gene Symbol AKAP13
Gene ID (NCBI) 11214
RRIDAB_2881017
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12802
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for AKAP13 antibody 27947-1-APDownload protocol
IHC protocol for AKAP13 antibody 27947-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols