AIFM3 Polyclonal antibody

AIFM3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 14778-1-AP

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Synonyms

AIFL, AIFM3, Apoptosis inducing factor 3



经过测试的应用

Positive WB detected inHEK-293 cells, U-87 MG cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14778-1-AP targets AIFM3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen AIFM3 fusion protein Ag6278 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name apoptosis-inducing factor, mitochondrion-associated, 3
Synonyms AIFL, AIFM3, Apoptosis inducing factor 3
Calculated Molecular Weight 67 kDa
Observed Molecular Weight 67 kDa
GenBank Accession NumberBC032485
Gene Symbol AIFM3
Gene ID (NCBI) 150209
RRIDAB_2258090
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96NN9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Apoptosis-inducing factor, mitochondrion-associated 3 (AIFM3) is a mitochondrial protein/flavoenzyme and its gene is located on chromosome 22q11.21. Mature human AIFM3 protein consists of 598 amino acids with a molecular weight of 66 kDa and is predominantly localized in the mitochondria. AIFM3 is widely expressed in many tissues, and breast cancer tissues and cholangiocarcinoma tissue (PMID: 32664187).

实验方案

Product Specific Protocols
WB protocol for AIFM3 antibody 14778-1-APDownload protocol
IHC protocol for AIFM3 antibody 14778-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols