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AIFM2/ FSP1 Polyclonal antibody

AIFM2/ FSP1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

IHC, IP, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 20886-1-AP

Print datasheet

Synonyms

AIFM2, AIFM2/ FSP1, AMID, Apoptosis inducing factor 2, ferroptosis suppressor protein 1, FSP1, p53 responsive gene 3 protein, PRG3, RP11 367H5.2



经过测试的应用

Positive WB detected inmouse kidney tissue, HeLa cells, rat liver tissue, HepG2 cells, PC-12 ceslls
Positive IP detected inL02 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy AIFM2 Ready-To-Use IHC Kit. AIFM2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20886-1-AP targets AIFM2/ FSP1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB, ELISA
Cited ApplicationsCoIP, IF, IHC, IP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, duck
Immunogen AIFM2/ FSP1 fusion protein Ag13412 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name apoptosis-inducing factor, mitochondrion-associated, 2
Synonyms AIFM2, AIFM2/ FSP1, AMID, Apoptosis inducing factor 2, ferroptosis suppressor protein 1, FSP1, p53 responsive gene 3 protein, PRG3, RP11 367H5.2
Calculated Molecular Weight 41 kDa
Observed Molecular Weight41 kDa
GenBank Accession NumberBC023601
Gene Symbol AIFM2
Gene ID (NCBI) 84883
RRIDAB_2878756
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BRQ8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The human AIFM2 protein (also known as FSP1 or AMID) is an apoptosis associated flavoprotein with a 6-hydroxy FAD cofactor. AIFM2 is a NAD(P)H-binding oxidoreductase with some sequence similarities to A1FM1 (formerly known as AIF, Apoptosis Inducing Factor), a mitochondrion-associated enzyme which relocates to the cell nucleus during apoptosis and is considered to be a key player in the progression of cell death.

实验方案

Product Specific Protocols
WB protocol for AIFM2/ FSP1 antibody 20886-1-APDownload protocol
IHC protocol for AIFM2/ FSP1 antibody 20886-1-APDownload protocol
IP protocol for AIFM2/ FSP1 antibody 20886-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Res

Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression

Authors - Shiqiao Qiu
humanWB

Nature

The CoQ oxidoreductase FSP1 acts parallel to GPX4 to inhibit ferroptosis.

Authors - Kirill Bersuker
humanWB,IHC

Drug Resist Updat

Upregulation of CoQ shifts ferroptosis dependence from GPX4 to FSP1 in acquired radioresistance

Authors - Xu Lin
mouseWB

Nat Commun

Targeting ferroptosis by poly(acrylic) acid coated Mn3O4 nanoparticles alleviates acute liver injury

Authors - Xinyi Shan
mouseWB

Nat Commun

A targetable LIFR-NF-κB-LCN2 axis controls liver tumorigenesis and vulnerability to ferroptosis.

Authors - Fan Yao
mouseIHC,IF

Nat Commun

Tartrate-resistant acid phosphatase 5 promotes pulmonary fibrosis by modulating β-catenin signaling.

Authors - Yinan Hu