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ADAP1 Polyclonal antibody

ADAP1 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 13911-1-AP

Print datasheet

Synonyms

ADAP1, ArfGAP with dual PH domains 1, CENTA1, Centaurin alpha 1, Cnt a1, GCS1L, p42IP4

验证数据展示

  • IHC staining of human gliomas using 13911-1-AP

    IHC staining of human gliomas using 13911-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13911-1-AP (ADAP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of human brain using 13911-1-AP

    WB analysis of human brain using 13911-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 13911-1-AP (ADAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 13911-1-AP

    IF Staining of MCF-7 using 13911-1-AP

    Immunofluorescent analysis of MCF-7 cells, using ADAP1 antibody 13911-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

经过测试的应用

Positive WB detected inhuman brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells, Hela cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13911-1-AP targets ADAP1 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen ADAP1 fusion protein Ag4898 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ArfGAP with dual PH domains 1
Synonyms ADAP1, ArfGAP with dual PH domains 1, CENTA1, Centaurin alpha 1, Cnt a1, GCS1L, p42IP4
Calculated Molecular Weight 374 aa, 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC033747
Gene Symbol ADAP1
Gene ID (NCBI) 11033
RRIDAB_2257889
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75689
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ADAP1 antibody 13911-1-APDownload protocol
IHC protocol for ADAP1 antibody 13911-1-APDownload protocol
IF protocol for ADAP1 antibody 13911-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols