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Alpha Actin Polyclonal antibody

Alpha Actin Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 23082-1-AP

Print datasheet

Synonyms

Alpha 1 actin, Alpha actin

验证数据展示

  • WB analysis of mouse heart using 23082-1-AP

    WB analysis of mouse heart using 23082-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 23082-1-AP (alpha Actin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 23082-1-AP

    IHC staining of human heart using 23082-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 23082-1-AP (alpha Actin antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with ACTC1-specific antibody included
  • IHC staining of human heart using 23082-1-AP

    IHC staining of human heart using 23082-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 23082-1-AP (alpha Actin antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with ACTC1-specific antibody included
  • FC experiment of C2C12 using 23082-1-AP

    FC experiment of C2C12 using 23082-1-AP

    1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human Alpha Actin (23082-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inmouse heart tissue
Positive IHC detected inhuman heart tissue, mouse skeletal muscle tissue, mouse heart tissue, mouse colon tissue, human small intestine tissue, human colon tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inC2C12 cells
Planning an IHC experiment? We recommend our IHCeasy ACTC1-specific Ready-To-Use IHC Kit. ACTC1-specific primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

The immunogen of 23082-1-AP is Alpha Actin Fusion Protein expressed in E. coli.

Tested Applications FC, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Immunogen Alpha Actin fusion protein Ag19482 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name actin, alpha, cardiac muscle 1
Synonyms Alpha 1 actin, Alpha actin
Calculated Molecular Weight 377 aa, 42 kDa
Observed Molecular Weight 42-45 kDa
GenBank Accession NumberBC009978
Gene Symbol ACTC1
Gene ID (NCBI) 70
RRIDAB_2879206
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP68032
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Alpha Actin antibody 23082-1-APDownload protocol
IHC protocol for Alpha Actin antibody 23082-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Circ Heart Fail

Phenotype and Physiological Significance of the Endocardial Smooth Muscle Cells in Human Failing Hearts.

Authors - Hideshi Okada
View Article