• Featured Product
  • KD/KO Validated

ACSS2 Polyclonal antibody

ACSS2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 16087-1-AP

Print datasheet

Synonyms

ACAS2, AceCS, ACS, ACSA, ACSS2, acyl CoA synthetase short chain family member 2



经过测试的应用

Positive WB detected inHEK-293 cells, mouse liver tissue, HepG2 cells, mouse brain tissue, rat liver tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16087-1-AP targets ACSS2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB, ELISA
Cited ApplicationsIF, IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen ACSS2 fusion protein Ag9121 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name acyl-CoA synthetase short-chain family member 2
Synonyms ACAS2, AceCS, ACS, ACSA, ACSS2, acyl CoA synthetase short chain family member 2
Calculated Molecular Weight 701 aa, 79 kDa
Observed Molecular Weight 79 kDa
GenBank Accession NumberBC010141
Gene Symbol ACSS2
Gene ID (NCBI) 55902
RRIDAB_2878216
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NR19
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Acetyl-CoA Synthetase 2 (ACSS2) is also named as acyl-CoA synthetase short-chain family member 2. Acetyl-CoA Synthetase 2 (ACSS2) is responsible for lipogenesis by synthesizing acetyl-CoA from acetate for lipogenesis and promotes acetylation in nuclear (PMID: 29444517). It is also required for cell migration and invasion in renal cell carcinoma (RCC) through the promotion of autophagy (PMID: 29281714). ACSS2 has two isoforms with the molecular weight of 78 and 80 kDa.

实验方案

Product Specific Protocols
WB protocol for ACSS2 antibody 16087-1-APDownload protocol
IHC protocol for ACSS2 antibody 16087-1-APDownload protocol
IP protocol for ACSS2 antibody 16087-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Cell

Acetyl-CoA Derived from Hepatic Peroxisomal β-Oxidation Inhibits Autophagy and Promotes Steatosis via mTORC1 Activation.

Authors - Anyuan He
WB

Plant Physiol

FDX5 deletion affects metabolism of algae during the different phases of S-deprivation.

Authors - Venkataramanan Subramanian
mouseWB

Antioxidants (Basel)

Bisdemethoxycurcumin Alleviates Dextran Sodium Sulfate-Induced Colitis via Inhibiting NLRP3 Inflammasome Activation and Modulating the Gut Microbiota in Mice

Authors - Jingfei Zhang
ratWB

Neurosci Lett

Anti-depressant effects of acupuncture: The insights from NLRP3 mediated pyroptosis and inflammation.

Authors - Yiping Chen
humanWB

Exp Ther Med

hsa-miR-15b-5p regulates the proliferation and apoptosis of human vascular smooth muscle cells by targeting the ACSS2/PTGS2 axis.

Authors - Shujie Gan
humanWB

Nat Metab

The SESAME complex regulates cell senescence through the generation of acetyl-CoA.

Authors - Wanping Chen
  • KD Validated