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ACSM3 Polyclonal antibody

ACSM3 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC, IF, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 10168-2-AP

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Synonyms

ACSM3, Butyrate CoA ligase 3, Protein SA homolog, SA, SAH



经过测试的应用

Positive WB detected inHEK-293 cells, mouse kidney tissue
Positive IHC detected inhuman kidney tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10168-2-AP targets ACSM3 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications FC, IF, IHC, WB, ELISA
Cited ApplicationsIF, IHC, WB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen ACSM3 fusion protein Ag0224 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name acyl-CoA synthetase medium-chain family member 3
Synonyms ACSM3, Butyrate CoA ligase 3, Protein SA homolog, SA, SAH
Calculated Molecular Weight 49 kDa, 66 kDa
Observed Molecular Weight 60-70 kDa
GenBank Accession NumberBC002790
Gene Symbol ACSM3
Gene ID (NCBI) 6296
RRIDAB_2222699
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ53FZ2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SA hypertension-associated rat homolog (SAH, synonym: SA) is expressed at 10-fold greater levels in the kidney of the spontaneously hypertensive rat than in the corresponding wild-type strain. The gene is linked to blood pressure levels in a number of crosses involving the spontaneously hypertensive rat and other strains of genetically hypertensive rats. Human SAH gene polymorphism may be associated with the renal prognosis of immunoglobulin A nephropathy through its effect on blood pressure. Furthermore, variation in SAH has a role in predisposition.

实验方案

Product Specific Protocols
WB protocol for ACSM3 antibody 10168-2-APDownload protocol
IHC protocol for ACSM3 antibody 10168-2-APDownload protocol
IF protocol for ACSM3 antibody 10168-2-APDownload protocol
FC protocol for ACSM3 antibody 10168-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,humanIHC

Cell

Organoid Models of Human and Mouse Ductal Pancreatic Cancer.

Authors - Sylvia F Boj
humanIHC

Front Cell Dev Biol

Predicting Panel of Metabolism and Immune-Related Genes for the Prognosis of Human Ovarian Cancer.

Authors - Lingyun Zhang
humanWB, IHC

Front Oncol

The Overexpression of Acyl-CoA Medium-Chain Synthetase-3 (ACSM3) Suppresses the Ovarian Cancer Progression via the Inhibition of Integrin β1/AKT Signaling Pathway.

Authors - Limei Yan
mouse

Sci Rep

Thioesterase induction by 2,3,7,8-tetrachlorodibenzo-p-dioxin results in a futile cycle that inhibits hepatic β-oxidation.

Authors - Giovan N Cholico
mouseWB,IF

Eur J Cell Biol

Proteomic and cell biological profiling of the renal phenotype of the mdx-4cv mouse model of Duchenne muscular dystrophy.

Authors - Paul Dowling
humanWB

Exp Ther Med

ACSM3 suppresses proliferation and induces apoptosis and cell cycle arrest in acute myeloid leukemia cells via the regulation of IGF2BP2

Authors - Xin Zheng