ACP1 Polyclonal antibody

ACP1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 22214-1-AP

Print datasheet

Synonyms

acid phosphatase 1, soluble, ACP1, Adipocyte acid phosphatase, HAAP, LMW PTP, LMW PTPase, Red cell acid phosphatase 1



经过测试的应用

Positive WB detected inmouse brain tissue, 4T1 cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

The immunogen of 22214-1-AP is ACP1 Fusion Protein expressed in E. coli.

Tested Applications IHC, WB,ELISA
Cited Applications IF
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen ACP1 fusion protein Ag17539 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name acid phosphatase 1, soluble
Synonyms acid phosphatase 1, soluble, ACP1, Adipocyte acid phosphatase, HAAP, LMW PTP, LMW PTPase, Red cell acid phosphatase 1
Calculated Molecular Weight 158 aa, 18 kDa
Observed Molecular Weight 18 kDa
GenBank Accession NumberBC007422
Gene Symbol ACP1
Gene ID (NCBI) 52
RRIDAB_2879032
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP24666
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human red cell acid phosphatase (ACP1) is a polymorphic enzyme closely related to cytosolic low molecular weight acid phosphatases, a protein family broadly conserved among eukaryotes. It catalyses the transfer of phosphate from phosphate ester substrates to suitable acceptor alcohols such as methanol and glycerol. This protein has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for ACP1 antibody 22214-1-APDownload protocol
IHC protocol for ACP1 antibody 22214-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nat Commun

Intracellular energy controls dynamics of stress-induced ribonucleoprotein granules

Authors - Tao Wang