ACOT9 Monoclonal antibody

ACOT9 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Rat, mouse and More (1)

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

CloneNo.

3F10G5

Cat No : 66532-1-Ig

Print datasheet

Synonyms

ACATE2, ACOT9, Acyl CoA thioester hydrolase 9, acyl CoA thioesterase 9, CGI 16, MT ACT48



经过测试的应用

Positive WB detected inHEK-293 cells, RAW 264.7 cells, Jurkat cells, HSC-T6 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ACOT9 Ready-To-Use IHC Kit. ACOT9 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

66532-1-Ig targets ACOT9 in WB, IHC, ELISA applications and shows reactivity with Human, Rat, mouse samples.

Tested Applications IHC, WB,ELISA
Cited Applications WB
Tested Reactivity Human, Rat, mouse
Cited Reactivityhuman
Immunogen ACOT9 fusion protein Ag8884 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name acyl-CoA thioesterase 9
Synonyms ACATE2, ACOT9, Acyl CoA thioester hydrolase 9, acyl CoA thioesterase 9, CGI 16, MT ACT48
Calculated Molecular Weight 24 kDa, 49 kDa
Observed Molecular Weight 43-50 kDa
GenBank Accession NumberBC012573
Gene Symbol ACOT9
Gene ID (NCBI) 23597
RRIDAB_2881895
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y305
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACOT9 (Acyl-coenzyme A thioesterase 9) is a member of the acyl-CoA thioesterase superfamily, which is a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. ACOT9 has 4 isoforms with the molecular mass of 43-50 kDa.

实验方案

Product Specific Protocols
WB protocol for ACOT9 antibody 66532-1-IgDownload protocol
IHC protocol for ACOT9 antibody 66532-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Adv Sci (Weinh)

SUCLG2 Regulates Mitochondrial Dysfunction through Succinylation in Lung Adenocarcinoma

Authors - Qifan Hu