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  • Featured Product
  • KD/KO Validated

ACAP3 Polyclonal antibody

ACAP3 Polyclonal Antibody for IF/ICC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, WB,ELISA

Conjugate

Unconjugated

Cat No : 17570-1-AP

Print datasheet

Synonyms

ACAP3, Centaurin beta 5, CENTB5, Cnt b5, KIAA1716

验证数据展示

  • WB analysis of Jurkat using 17570-1-AP

    WB analysis of Jurkat using 17570-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 17570-1-AP (ACAP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of SH-SY5Y using 17570-1-AP

    IF Staining of SH-SY5Y using 17570-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed SH-SY5Y cells using 17570-1-AP (ACAP3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inJurkat cells, SH-SY5Y cells
Positive IF detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17570-1-AP targets ACAP3 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, WB,ELISA
Cited Applications IF, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen ACAP3 fusion protein Ag11777 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ArfGAP with coiled-coil, ankyrin repeat and PH domains 3
Synonyms ACAP3, Centaurin beta 5, CENTB5, Cnt b5, KIAA1716
Calculated Molecular Weight 834 aa, 92 kDa
Observed Molecular Weight 92 kDa
GenBank Accession NumberBC047001
Gene Symbol ACAP3
Gene ID (NCBI) 116983
RRIDAB_2257668
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96P50
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ACAP3 antibody 17570-1-APDownload protocol
IF protocol for ACAP3 antibody 17570-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IF

Biochem J

ACAP3 regulates neurite outgrowth through its GAP activity specific to Arf6 in mouse hippocampal neurons.

Authors - Yuki Miura
View Article
mouseWB

Biochem Biophys Res Commun

ACAP3, the GTPase-activating protein specific to the small GTPase Arf6, regulates neuronal migration in the developing cerebral cortex.

Authors - Yuki Miura
  • KD Validated
View Article