ACAP1 Monoclonal antibody

ACAP1 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B4C9

Cat No : 66596-1-Ig

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Synonyms

ACAP1, Centaurin beta 1, CENTB1, Cnt b1, KIAA0050



经过测试的应用

Positive WB detected inJurkat cells, HL-60 cells, K-562 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

66596-1-Ig targets ACAP1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, WB, ELISA
Cited Applications WB
Tested Reactivity Human
Cited Reactivityhuman
Immunogen ACAP1 fusion protein Ag14254 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ArfGAP with coiled-coil, ankyrin repeat and PH domains 1
Synonyms ACAP1, Centaurin beta 1, CENTB1, Cnt b1, KIAA0050
Calculated Molecular Weight 740 aa, 82 kDa
Observed Molecular Weight 81 kDa
GenBank Accession NumberBC018543
Gene Symbol ACAP1
Gene ID (NCBI) 9744
RRIDAB_2881956
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ15027
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACAP1, also named as Centaurin-beta-1, is a 740 amino acid protein, which is expressed Highly in lung and spleen. ACAP1 as a GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) is required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface and required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.

实验方案

Product Specific Protocols
WB protocol for ACAP1 antibody 66596-1-IgDownload protocol
IHC protocol for ACAP1 antibody 66596-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancers (Basel)

ACAP1 Deficiency Predicts Inferior Immunotherapy Response in Solid Tumors

Authors - Qiyi Yi