Aggrecan Polyclonal antibody

Aggrecan Polyclonal Antibody for FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (4)

Applications

FC, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 13880-1-AP

Print datasheet

Synonyms

ACAN, AGC1, AGCAN, aggrecan, CSPG1, CSPGCP, MSK16, SEDK



经过测试的应用

Positive IHC detected inhuman lung tissue, human lung cancer tissue, human cartilage tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inATDC-5 cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13880-1-AP targets Aggrecan in IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IHC, ELISA
Cited Applications IF, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, rabbit, zebrafish, pig, canine
Immunogen Aggrecan fusion protein Ag4985 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name aggrecan
Synonyms ACAN, AGC1, AGCAN, aggrecan, CSPG1, CSPGCP, MSK16, SEDK
Calculated Molecular Weight 250 kDa
GenBank Accession NumberBC036445
Gene Symbol ACAN
Gene ID (NCBI) 176
RRIDAB_2722780
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP16112
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Aggrecan (ACAN), also named as CSPCP, AGC1 and MSK16, belongs to the aggrecan/versican proteoglycan family (also including versican, brevican and neurocan). It is a major component of extracellular matrix of cartilaginous tissues. A major function of aggrecan is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.

实验方案

Product Specific Protocols
IHC protocol for Aggrecan antibody 13880-1-APDownload protocol
FC protocol for Aggrecan antibody 13880-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseIHC,IF

Nature

A plant-derived natural photosynthetic system for improving cell anabolism

Authors - Pengfei Chen
human

Sci Transl Med

Single-cell atlas of human infrapatellar fat pad and synovium implicates APOE signaling in osteoarthritis pathology

Authors - Su'an Tang
human

J Extracell Vesicles

miR-23a-3p-abundant small extracellular vesicles released from Gelma/nanoclay hydrogel for cartilage regeneration.

Authors - Hongxing Hu
mouse,ratIF

Cell Death Differ

Developmental growth plate cartilage formation suppressed by artificial light at night via inhibiting BMAL1-driven collagen hydroxylation

Authors - Guangjin Chen
mouse

Nat Commun

Mutant Huntingtin stalls ribosomes and represses protein synthesis in a cellular model of Huntington disease.

Authors - Mehdi Eshraghi
human

EBioMedicine

CircRNA-CIDN mitigated compression loading-induced damage in human nucleus pulposus cells via miR-34a-5p/SIRT1 axis.

Authors - Qian Xiang