ABCF2 Polyclonal antibody

ABCF2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF, IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 10226-1-AP

Print datasheet

Synonyms

ABC28, ABCF2, EST133090, HUSSY 18, HUSSY18, M ABC1



经过测试的应用

Positive WB detected inNIH/3T3 cells, HEK-293 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

10226-1-AP targets ABCF2 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF, IHC, IP, WB,ELISA
Cited Applications WB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen ABCF2 fusion protein Ag0310 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATP-binding cassette, sub-family F (GCN20), member 2
Synonyms ABC28, ABCF2, EST133090, HUSSY 18, HUSSY18, M ABC1
Calculated Molecular Weight 71 kDa
Observed Molecular Weight 71 kDa
GenBank Accession NumberBC001661
Gene Symbol ABCF2
Gene ID (NCBI) 10061
RRIDAB_2304888
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UG63
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ABCF2 (ABC28, M-ABC1, HUSSY-18, EST133090, DKFZp586K1823) is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins are composed of transmembrane domains (TMDs), and nucleotide binding domains (NBDs, or ATP-binding cassettes, ABSs). Based on their sequence similarity scores, the members of the human ABC protein family can be grouped into eight subfamilies, including the MDR/TAP, the ALD, the MRP/CFTR, the ABC1, the White, the RNAseL inhibitor, the ANSA, and the GCN20 subfamilies. ABCF2 is a member of the GCN20 subfamily. ABC proteins transport various molecules across extra- and intracellular membranes.

实验方案

Product Specific Protocols
WB protocol for ABCF2 antibody 10226-1-APDownload protocol
IHC protocol for ABCF2 antibody 10226-1-APDownload protocol
IF protocol for ABCF2 antibody 10226-1-APDownload protocol
IP protocol for ABCF2 antibody 10226-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

Purification of cross-linked RNA-protein complexes by phenol-toluol extraction.

Authors - Erika C Urdaneta