Various lysates were subjected to SDS PAGE followed by western blot with 66546-1-Ig (XRCC5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66546-1-PBS in a different storage buffer formulation.
IP result of anti-XRCC5 (IP:66546-1-Ig, 5ug; Detection:66546-1-Ig 1:20000) with HeLa cells lysate 3200 ug. This data was developed using the same antibody clone with 66546-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66546-1-Ig (XRCC5 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66546-1-PBS in a different storage buffer formulation.
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 66546-1-Ig (XRCC5 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66546-1-PBS in a different storage buffer formulation.
IP result of anti-XRCC5 (IP:66546-1-Ig, 5ug; Detection:66546-1-Ig 1:40000) with HeLa cells lysate 640 ug. This data was developed using the same antibody clone with 66546-1-PBS in a different storage buffer formulation.