Purity of recombinant human Cystatin C was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Recombinant Human Cystatin C (HZ-1211) inhibits papain protease activity in a dose-dependent manner. Papain protease activity was measured by colorimetric assay using L-BAPA as the substrate. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range form 0.5-2.6 µg/mL.

Purity of GMP recombinant human Cystatin C was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

Recombinant Human GMP Cystatin C (HZ-1211-GMP) inhibits papain protease activity in a dose-dependent manner. Papain protease activity was measured by colorimetric assay using L-BAPA as the substrate. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values range form 0.5-2.6 µg/mL.