RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 28058-1-AP (Cd68 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 28058-1-AP (Cd68 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 28058-1-AP (Cd68 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using Cd68 antibody (28058-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using Cd68 antibody (28058-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
1X10^6 RAW 264.7 cells were intracellularly stained with 0.4 ug Anti-Mouse Cd68 (28058-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).