MAP2 Monoclonal antibody
MAP2 Monoclonal Antibody for FC, IF, IHC, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human, mouse, rat and More (1)
Applications
FC, IF, IHC, ELISA and More (2)
Conjugate
Unconjugated
CloneNo.
1C3E6
验证数据展示
经过测试的应用
Positive IHC detected in | mouse brain tissue, rat cerebellum tissue, rat brain tissue, human brain tissue, mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | rat brain tissue, mouse brain tissue |
Positive FC detected in | SH-SY5Y cells, Neuro-2a cells |
推荐稀释比
Application | Dilution |
---|---|
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
67015-1-Ig targets MAP2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Applications | FC, IF, IHC, ELISA |
Cited Applications | IF, IHC, IP, WB |
Tested Reactivity | Human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | MAP2 fusion protein Ag11349 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | microtubule-associated protein 2 |
Synonyms | MAP 2, MAP2, MAP2A, MAP2B, MAP2C |
Calculated Molecular Weight | 200 kDa |
GenBank Accession Number | BC038857 |
Gene Symbol | MAP2 |
Gene ID (NCBI) | 4133 |
RRID | AB_2882331 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P11137 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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IHC protocol for MAP2 antibody 67015-1-Ig | Download protocol |
IF protocol for MAP2 antibody 67015-1-Ig | Download protocol |
FC protocol for MAP2 antibody 67015-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Stem Cell In vivo reprogramming of NG2 glia enables adult neurogenesis and functional recovery following spinal cord injury.
| ||
Small Stemness Maintenance and Massproduction of Neural Stem Cells on Poly L-Lactic Acid Nanofibrous Membrane Based on Piezoelectriceffect. | ||
Cell Death Dis ChemR23 activation attenuates cognitive impairment in chronic cerebral hypoperfusion by inhibiting NLRP3 inflammasome-induced neuronal pyroptosis | ||
Cell Death Dis Pyruvate dehydrogenase kinase 1 protects against neuronal injury and memory loss in mouse models of diabetes | ||
Acta Neuropathol Commun TMEM106B deficiency impairs cerebellar myelination and synaptic integrity with Purkinje cell loss. |