CD8a Monoclonal antibody

CD8a Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

IF, IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

1G2B10

Cat No : 66868-1-Ig

Print datasheet

Synonyms

CD8, CD8A, CD8a molecule, Leu2, MAL, p32



经过测试的应用

Positive WB detected inMOLT-4 cells, human peripheral blood leukocyte
Positive IHC detected inhuman tonsillitis tissue, human appendicitis tissue, human pancreas cancer tissue, human colon cancer tissue, human breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue
Planning an IHC experiment? We recommend our IHCeasy CD8 Ready-To-Use IHC Kit. CD8 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:10000-1:40000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66868-1-Ig targets CD8a in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human samples.

Tested Applications IF, IHC, WB, ELISA
Cited Applications FC, IF, IHC, WB
Tested Reactivity Human
Cited Reactivityhuman
Immunogen CD8a fusion protein Ag11111 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name CD8a molecule
Synonyms CD8, CD8A, CD8a molecule, Leu2, MAL, p32
Calculated Molecular Weight 235 aa, 26 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC025715
Gene Symbol CD8A
Gene ID (NCBI) 925
ENSEMBL Gene IDENSG00000153563
RRIDAB_2882205
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP01732
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD8 is a transmembrane glycoprotein that is predominantly expressed on the surface of cytotoxic T cells, and can also be found on natural killer cells, cortical thymocytes, and dendritic cells. CD8 serves as a co-receptor for the T cell receptor (TCR). Both CD8 and TCR recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. CD8 plays a role in T cell development and activation of mature T cells. This antibody is raised against 23-183 aa of human CD8a, the alpha chain of CD8 molecule.

实验方案

Product Specific Protocols
WB protocol for CD8a antibody 66868-1-IgDownload protocol
IHC protocol for CD8a antibody 66868-1-IgDownload protocol
IF protocol for CD8a antibody 66868-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IF

ACS Nano

Tailoring Chemoimmunostimulant Bioscaffolds for Inhibiting Tumor Growth and Metastasis after Incomplete Microwave Ablation.

Authors - Yuting Shen
humanIF

J Med Virol

Combination of novel oncolytic herpesvirus with paclitaxel as an efficient strategy for breast cancer therapy

Authors - Xinyue Deng
humanIF

Cell Death Differ

METTL3 inhibition induced by M2 macrophage-derived extracellular vesicles drives anti-PD-1 therapy resistance via M6A-CD70-mediated immune suppression in thyroid cancer

Authors - Junya Ning
humanIHC

Nat Commun

Single-cell transcriptomics links malignant T cells to the tumor immune landscape in cutaneous T cell lymphoma.

Authors - Xiangjun Liu
humanIF

J Exp Clin Cancer Res

FAM171B stabilizes vimentin and enhances CCL2-mediated TAM infiltration to promote bladder cancer progression

Authors - Wei-Min Hu
humanIF,FC

J Immunother Cancer

Interleukin-10 induces expression of CD39 on CD8+T cells to potentiate anti-PD1 efficacy in EGFR-mutated non-small cell lung cancer

Authors - Meng Qiao