ROCK1 Monoclonal antibody

ROCK1 Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

3G5E12

Cat No : 66782-1-Ig

Print datasheet

Synonyms

p160 ROCK 1, P160ROCK, PRO0435, ROCK1



经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

66782-1-Ig targets ROCK1 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB,ELISA
Cited Applications WB
Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman, mouse
Immunogen ROCK1 fusion protein Ag16317 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name Rho-associated, coiled-coil containing protein kinase 1
Synonyms p160 ROCK 1, P160ROCK, PRO0435, ROCK1
Calculated Molecular Weight 1354 aa, 158 kDa
Observed Molecular Weight 150-160 kDa
GenBank Accession NumberBC113114
Gene Symbol ROCK1
Gene ID (NCBI) 6093
RRIDAB_2882127
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13464
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ROCK1, also named as p160ROCK and NY-REN-35, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. ROCK1 phosphorylates and activates DAPK3, which then regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. ROCK1 is required for centromere positioning and centromere-dependent exit from mitosis. It is necessary for apoptotic membrane blebbing. ROCK1 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein.

实验方案

Product Specific Protocols
WB protocol for ROCK1 antibody 66782-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Cell Biochem

Lnc-MUC20-9 binds to ROCK1 and functions as a tumor suppressor in bladder cancer.

Authors - Ranran Dai
mouseWB

Am J Transl Res

β-catenin regulates myocardial ischemia/reperfusion injury following heterotopic heart transplantation in mice by modulating PTEN pathways.

Authors - Qian Ban
mouseWB

Food Funct

Strains producing different short-chain fatty acids alleviate DSS-induced ulcerative colitis by regulating intestinal microecology

Authors - Shuo Yang