CRM1 Monoclonal antibody

CRM1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (3)

Applications

IF, IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

2D3B6

Cat No : 66763-1-Ig

Print datasheet

Synonyms

CRM1, DKFZp686B1823, Exp1, Exportin 1, XPO1



经过测试的应用

Positive WB detected inA431 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, RAW 264.7 cells, HEK-293 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CRM1 Ready-To-Use IHC Kit. CRM1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66763-1-Ig targets CRM1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IF, IHC, WB, ELISA
Cited Applications IHC, IP, WB
Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman, mouse, rat
Immunogen CRM1 fusion protein Ag27337 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name exportin 1 (CRM1 homolog, yeast)
Synonyms CRM1, DKFZp686B1823, Exp1, Exportin 1, XPO1
Calculated Molecular Weight 1071 aa, 123 kDa
Observed Molecular Weight 100-110 kDa
GenBank Accession NumberBC032847
Gene Symbol XPO1
Gene ID (NCBI) 7514
RRIDAB_2882109
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14980
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CRM1 (chromosome region maintenance 1, also known as exportin-1 or XPO1), a member of the karyopherin β family of transport receptors, is an important nuclear export receptor recognizing proteins bearing a leucine-rich nuclear export signal (PMID: 9323133). CRM1 is the major receptor for the export of proteins out of the nucleus and is also required for transport of many RNAs (PMID: 17317185). In addition to nuclear export, CRM1 also plays a role in centrosome duplication and spindle assembly (PMID: 22773955). Elevated CRM1 expression has been reported in several cancers, which implicates CRM1 as a potential anti-cancer drug target (PMID: 22677130; 21683812 ).

实验方案

Product Specific Protocols
WB protocol for CRM1 antibody 66763-1-IgDownload protocol
IHC protocol for CRM1 antibody 66763-1-IgDownload protocol
IF protocol for CRM1 antibody 66763-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,ratWB

Diabetologia

Saturated fatty acids entrap PDX1 in stress granules and impede islet beta cell function.

Authors - Mu Zhang
WB

Sci Rep

Anti-tumor efficacy of Selinexor (KPT-330) in gastric cancer is dependent on nuclear accumulation of p53 tumor suppressor.

Authors - Vinod Vijay Subhash
mouseWB

Exp Gerontol

Age-dependent changes in nuclear-cytoplasmic signaling in skeletal muscle.

Authors - Shama R Iyer
humanWB

Exp Cell Res

NEDD4 protects vascular endothelial cells against Angiotensin II-induced cell death via enhancement of XPO1-mediated nuclear export.

Authors - Jianning Xu
humanIHC

PeerJ

Hub gene associated with prognosis in bladder cancer is a novel therapeutic target

Authors - Dengpan Fang
humanWB

Cell Death Dis

Synergistic antifibrotic effects of miR-451 with miR-185 partly by co-targeting EphB2 on hepatic stellate cells.

Authors - Xiaogang Chen