ERp57/ERp60 Monoclonal antibody
ERp57/ERp60 Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
IF, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
3G4G7
验证数据展示
经过测试的应用
Positive WB detected in | U2OS cells, HeLa cells, HEK-293 cells, Jurkat cells, RAW 264.7 cells, LNCaP cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells |
Positive IHC detected in | human lung cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HEK-293 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:20000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Immunofluorescence (IF) | IF : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66423-1-Ig targets ERp57/ERp60 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF, IHC, WB, ELISA |
Tested Reactivity | human, mouse, rat |
Immunogen | ERp57/ERp60 fusion protein Ag8741 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | protein disulfide isomerase family A, member 3 |
Synonyms | 58 kDa microsomal protein, Cell and organelle markers, Disulfide isomerase ER 60, endoplasmic reticulum marker, ER protein 57, ER protein 60, ER60, ERp57, ERp60, ERp61, GRP57, GRP58, HsT17083, P58, PDIA3, PI PLC, Protein disulfide isomerase A3 |
Calculated Molecular Weight | 505 aa, 57 kDa |
Observed Molecular Weight | 57 kDa, 60 kDa |
GenBank Accession Number | BC014433 |
Gene Symbol | PDIA3 |
Gene ID (NCBI) | 2923 |
RRID | AB_2881795 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P30101 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for ERp57/ERp60 antibody 66423-1-Ig | Download protocol |
IHC protocol for ERp57/ERp60 antibody 66423-1-Ig | Download protocol |
IF protocol for ERp57/ERp60 antibody 66423-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |