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CDK6 Monoclonal antibody

CDK6 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF, WB, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

4B9C11

Cat No : 66278-1-Ig

Print datasheet

Synonyms

CDK6, Cell division protein kinase 6, cyclin dependent kinase 6, PLSTIRE



经过测试的应用

Positive WB detected inHEK-293 cells, COLO 320 cells, Jurkat cells, THP-1 cells, ROS1728 cells, HeLa cells, NIH/3T3 cells, HepG2 cells, K-562 cells, HSC-T6 cells, C6 cells, Raw 264.7 cells
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66278-1-Ig targets CDK6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, WB, ELISA
Cited Applications IF, IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen CDK6 fusion protein Ag5600 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cyclin-dependent kinase 6
Synonyms CDK6, Cell division protein kinase 6, cyclin dependent kinase 6, PLSTIRE
Calculated Molecular Weight 326 aa, 36 kDa
Observed Molecular Weight 36-40 kDa
GenBank Accession NumberBC027989
Gene Symbol CDK6
Gene ID (NCBI) 1021
RRIDAB_2881661
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ00534
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDK6(Cyclin-dependent kinase 6) is involved in initiation and maintenance of cell cycle exit during cell differentiation and it prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types.The molecular weight of CDK6 is 36-40 KDa (PMID: 23563707, 8114739). 

实验方案

Product Specific Protocols
WB protocol for CDK6 antibody 66278-1-IgDownload protocol
IF protocol for CDK6 antibody 66278-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Phytomedicine

Transcriptomics and molecular docking reveal the potential mechanism of lycorine against pancreatic cancer

Authors - Xin Zhou
humanWB

Int J Mol Sci

(20S) Ginsenoside Rh2 Exerts Its Anti-Tumor Effect by Disrupting the HSP90A-Cdc37 System in Human Liver Cancer Cells.

Authors - Chen Chen
humanWB

Cell Oncol (Dordr)

LINC02154 promotes the proliferation and metastasis of hepatocellular carcinoma by enhancing SPC24 promoter activity and activating the PI3K-AKT signaling pathway.

Authors - Huan Yue
humanWB

Oxid Med Cell Longev

MICAL2 Contributes to Gastric Cancer Cell Proliferation by Promoting YAP Dephosphorylation and Nuclear Translocation

Authors - Chenxiang Qi
humanWB

Int J Oncol

GBP1 promotes erlotinib resistance via PGK1‑activated EMT signaling in non‑small cell lung cancer.

Authors - Lifang Cheng
humanWB

Cell Signal

circ_ARF3 regulates the pathogenesis of osteosarcoma by sponging miR-1299 to maintain CDK6 expression.

Authors - Ai-Mei Gao