PD-1/CD279 Monoclonal antibody

PD-1/CD279 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat, mouse, pig

Applications

FC, IF, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

4H4D1

Cat No : 66220-1-Ig

Print datasheet

Synonyms

CD279, hPD 1, hPD l, PD1, PD-1, PD-1/CD279, PDCD1, programmed cell death 1, Protein PD 1, SLEB2



经过测试的应用

Positive WB detected inRAW 264.7 cells, human lymph node tissue, rat spleen tissue, mouse thymus tissue, Jurkat cells, MOLT-4 cells, THP-1 cells, CTLL-2 cells, pig thymus tissue
Positive IHC detected inhuman tonsillitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue, human lymphoma tissue
Positive FC detected inPMA and ionomycin treated MOLT-4 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66220-1-Ig targets PD-1/CD279 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, rat, mouse, pig samples.

Tested Applications FC, IF, IHC, WB, ELISA
Cited ApplicationsFC, IF, IHC, WB
Tested Reactivity human, rat, mouse, pig
Cited Reactivityhuman, mouse, rat
Immunogen PD-1/CD279 fusion protein Ag12470 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name programmed cell death 1
Synonyms CD279, hPD 1, hPD l, PD1, PD-1, PD-1/CD279, PDCD1, programmed cell death 1, Protein PD 1, SLEB2
Calculated Molecular Weight 288 aa, 32 kDa
Observed Molecular Weight 32 kDa, 47-55 kDa
GenBank Accession NumberBC074740
Gene Symbol PDCD1
Gene ID (NCBI) 5133
RRIDAB_2881611
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15116
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436). The calculated molecular weight of PD-1 is 32 kDa. It has been reported that PD-1 is heavily glycosylated and migrates with an apparent molecular mass of 47-55 kDa on SDS-PAGE (PMID: 8671665; 17640856; 17003438).

实验方案

Product Specific Protocols
WB protocol for PD-1/CD279 antibody 66220-1-IgDownload protocol
IHC protocol for PD-1/CD279 antibody 66220-1-IgDownload protocol
IF protocol for PD-1/CD279 antibody 66220-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseIHC,IF

Nat Commun

ERK and USP5 govern PD-1 homeostasis via deubiquitination to modulate tumor immunotherapy

Authors - Xiangling Xiao
humanWB

Nat Commun

m6A mRNA demethylase FTO regulates melanoma tumorigenicity and response to anti-PD-1 blockade.

Authors - Seungwon Yang
mouseWB

Cancers (Basel)

Overcoming PD-1 Inhibitor Resistance with a Monoclonal Antibody to Secreted Frizzled-Related Protein 2 in Metastatic Osteosarcoma.

Authors - Patrick Nasarre
human,mouseWB,IHC

Biochem Pharmacol

Targeting HMGA1 contributes to immunotherapy in aggressive breast cancer while suppressing EMT

Authors - Xing Chang
humanWB

Aging (Albany NY)

Elevated lymphocyte specific protein 1 expression is involved in the regulation of leukocyte migration and immunosuppressive microenvironment in glioblastoma.

Authors - Jing-Yuan Cao
mouseWB, IF

CNS Neurosci Ther

The immunomodulatory effect of microglia on ECM neuroinflammation via the PD-1/PD-L1 pathway.

Authors - Yan Shen