PD-1/CD279 Monoclonal antibody
PD-1/CD279 Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, rat, mouse, pig
Applications
FC, IF, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
4H4D1
验证数据展示
经过测试的应用
Positive WB detected in | RAW 264.7 cells, human lymph node tissue, rat spleen tissue, mouse thymus tissue, Jurkat cells, MOLT-4 cells, THP-1 cells, CTLL-2 cells, pig thymus tissue |
Positive IHC detected in | human tonsillitis tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | human tonsillitis tissue, human lymphoma tissue |
Positive FC detected in | PMA and ionomycin treated MOLT-4 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66220-1-Ig targets PD-1/CD279 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, rat, mouse, pig samples.
Tested Applications | FC, IF, IHC, WB, ELISA |
Cited Applications | FC, IF, IHC, WB |
Tested Reactivity | human, rat, mouse, pig |
Cited Reactivity | human, mouse, rat |
Immunogen | PD-1/CD279 fusion protein Ag12470 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | programmed cell death 1 |
Synonyms | CD279, hPD 1, hPD l, PD1, PD-1, PD-1/CD279, PDCD1, programmed cell death 1, Protein PD 1, SLEB2 |
Calculated Molecular Weight | 288 aa, 32 kDa |
Observed Molecular Weight | 32 kDa, 47-55 kDa |
GenBank Accession Number | BC074740 |
Gene Symbol | PDCD1 |
Gene ID (NCBI) | 5133 |
RRID | AB_2881611 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q15116 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for PD-1/CD279 antibody 66220-1-Ig | Download protocol |
IHC protocol for PD-1/CD279 antibody 66220-1-Ig | Download protocol |
IF protocol for PD-1/CD279 antibody 66220-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Commun ERK and USP5 govern PD-1 homeostasis via deubiquitination to modulate tumor immunotherapy | ||
Nat Commun m6A mRNA demethylase FTO regulates melanoma tumorigenicity and response to anti-PD-1 blockade. | ||
Cancers (Basel) Overcoming PD-1 Inhibitor Resistance with a Monoclonal Antibody to Secreted Frizzled-Related Protein 2 in Metastatic Osteosarcoma. | ||
Biochem Pharmacol Targeting HMGA1 contributes to immunotherapy in aggressive breast cancer while suppressing EMT | ||
Aging (Albany NY) Elevated lymphocyte specific protein 1 expression is involved in the regulation of leukocyte migration and immunosuppressive microenvironment in glioblastoma. | ||
CNS Neurosci Ther The immunomodulatory effect of microglia on ECM neuroinflammation via the PD-1/PD-L1 pathway. |