- Featured Product
- KD/KO Validated
Cyclin D1 Monoclonal antibody
Cyclin D1 Monoclonal Antibody for FC, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat and More (4)
Applications
FC, WB, ELISA and More (3)
Conjugate
Unconjugated
637
CloneNo.
2G3G5
验证数据展示
经过测试的应用
Positive WB detected in | U2OS cells, A549 cells, HeLa cells, MCF-7 cells, NIH/3T3 cells, RAW 264.7 cells, SH-SY5Y cells, A431 cells, SW 1990 cells, HepG2 cells, HSC-T6 cells, PC-12 cells, HCT 116 cells, SK-N-SH cells |
Positive FC detected in | SH-SY5Y cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:20000 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
60186-1-Ig targets Cyclin D1 in WB,FC,ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | FC, WB, ELISA |
Cited Applications | WB,IP,IHC,IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, goat, zebrafish, hamster, bovine |
Immunogen | Cyclin D1 fusion protein Ag0689 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | cyclin D1 |
Synonyms | B cell lymphoma 1 protein, CCND1, cyclin D, cyclin D1, D11S287E, G1/S specific cyclin D1, PRAD1, PRAD1 oncogene, U21B31 |
Calculated Molecular Weight | 295 aa, 34 kDa |
Observed Molecular Weight | 34 kDa |
GenBank Accession Number | BC000076 |
Gene Symbol | CCND1 |
Gene ID (NCBI) | 595 |
RRID | AB_10793718 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P24385 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Cyclin D1 antibody 60186-1-Ig | Download protocol |
FC protocol for Cyclin D1 antibody 60186-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Cell Biol TRIM15 and CYLD regulate ERK activation via lysine-63-linked polyubiquitination. | ||
Acta Pharm Sin B The substitution of SERCA2 redox cysteine 674 promotes pulmonary vascular remodeling by activating IRE1α/XBP1s pathway. | ||
J Clin Invest Circular RNA circEsyt2 regulates vascular smooth muscle cell remodeling via splicing regulation. | ||
EBioMedicine A miR-567-PIK3AP1-PI3K/AKT-c-Myc feedback loop regulates tumour growth and chemoresistance in gastric cancer. |