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  • Featured Product
  • KD/KO Validated

SHH Polyclonal antibody

SHH Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

FC, IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 20697-1-AP

Print datasheet

Synonyms

HHG 1, HHG1, HLP3, HPE3, MCOPCB5, SHH, SMMCI, Sonic hedgehog protein, TPT, TPTPS

验证数据展示

  • WB analysis using 20697-1-AP

    WB analysis using 20697-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20697-1-AP (SHH antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 20697-1-AP

    IHC staining of human liver cancer using 20697-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20697-1-AP (SHH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 20697-1-AP

    IHC staining of human liver cancer using 20697-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20697-1-AP (SHH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 20697-1-AP

    IHC staining of human pancreas cancer using 20697-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 20697-1-AP (SHH antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary tumor using 20697-1-AP

    IHC staining of human ovary tumor using 20697-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20697-1-AP (SHH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse embryo using 20697-1-AP

    IHC staining of mouse embryo using 20697-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 20697-1-AP (SHH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 20697-1-AP

    IF Staining of HepG2 using 20697-1-AP

    Immunofluorescent analysis of HepG2 cells, using SHH antibody 20697-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IF Staining of mouse kidney using 20697-1-AP

    IF Staining of mouse kidney using 20697-1-AP

    Frozen tissue section of adult mouse kidney was stained for acetylated ɑ-tubulin (magenta, Cat. No CL488-66200), CD31/PECAM-1 (white), and Shh (green, Cat. No 20697-1-AP) with DAPI as a counterstain for visualizing the nucleus (blue). acetylated ɑ-tubulin stains primary cilia and was conjugated to Coralite-488 fluorescent dye and psedocolored to magenta. CD31 stains endocardial/endothelial cells and was visualized with an Alexa Fluor 647 secondary antibody and psedocolored to white. Shh stains Sonic Hedgehog and was visualized with an Alexa Fluor 555 secondary antibody and psedocolored to green.The Image and figure legends are intellectual property of @LAF_in_the_LAB.

  • IP experiment of mouse liver using 20697-1-AP

    IP experiment of mouse liver using 20697-1-AP

    IP result of anti-SHH (IP:20697-1-AP, 4ug; Detection:20697-1-AP 1:500) with mouse liver tissue lysate 6400ug.

  • WB analysis using 20697-1-AP

    WB analysis using 20697-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20697-1-AP (SHH antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using 20697-1-AP

    FC experiment of HepG2 using 20697-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human SHH (20697-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHeLa cells, rat kidney tissue, HepG2 cells, LNCaP cells, PC-3 cells, mouse liver tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver cancer tissue, mouse kidney tissue, human pancreas cancer tissue, human ovary tumor tissue, mouse embryo tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, mouse kidney tissue
Positive FC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20697-1-AP targets SHH in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsIF, IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, mink
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sonic hedgehog homolog (Drosophila)
Synonyms HHG 1, HHG1, HLP3, HPE3, MCOPCB5, SHH, SMMCI, Sonic hedgehog protein, TPT, TPTPS
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 45-51 kDa
GenBank Accession NumberNM_000193
Gene Symbol SHH
Gene ID (NCBI) 6469
RRIDAB_10694828
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15465
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sonic hedgehog (SHH) is a member of the Hedgehog (Hh) family of proteins that control cell growth, survival, 

and fate.


What is the molecular weight of SHH?

The molecular weight of SHH is approximately 45 kDa, but it is cleaved autocatalytically to generate a 20 kDa 

N-terminal fragment and a 25 kDa C-terminal fragment responsible for hedgehog biological activity and the 

auto-processing machinery respectively (PMID: 10753901).


What is the tissue specificity of SHH?

SHH is the most broadly expressed mammalian Hh signaling molecule. During early vertebrate development, 

SHH is expressed in midline tissues such as the node, notochord, and floor plate to control the left-right and 

dorso-ventral patterning of the embryo (PMID: 12729563). SHH is expressed in fetal intestine, liver, kidney, 

and lung tissue, but is not expressed in adult tissues.


What is the cellular localization of SHH?

SHH is localized to the cell membrane. The N-terminal fragment is associated with lipid rafts at the cell surface, 

whereas the C-terminal fragment diffuses from the cell.


What is the role of SHH in the Sonic hedgehog signaling pathway?

This pathway is one of the major trafficking networks that regulate key events during developmental growth 

and patterning (PMID: 19368798). Loss of SHH signaling and regulation has been implicated in birth defects, 

tissue regeneration, stem cell renewal, and cancer growth (PMID: 15549094). The regulatory action of the SHH 

pathway is linked to the secretion, uptake, and translocation of the SHH protein; a ligand precursor, which is 

implicated in the regulation of the central nervous system polarity and neural patterning (PMID: 11130178, 

11801325).


实验方案

Product Specific Protocols
WB protocol for SHH antibody 20697-1-APDownload protocol
IHC protocol for SHH antibody 20697-1-APDownload protocol
IF protocol for SHH antibody 20697-1-APDownload protocol
IP protocol for SHH antibody 20697-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Nat Commun

TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling.

Authors - Rongxuan Zhu
View Article
mouseIHC

Theranostics

Single-cell Transcriptome Profiling reveals Dermal and Epithelial cell fate decisions during Embryonic Hair Follicle Development.

Authors - Wei Ge
View Article
humanWB

Cancer Lett

An SGLT2 inhibitor modulates SHH expression by activating AMPK to inhibit the migration and induce the apoptosis of cervical carcinoma cells.

Authors - Zipeng Xie
  • KD Validated
View Article
humanWB,IF

Oxid Med Cell Longev

TLR4 Modulates Senescence and Paracrine Action in Placental Mesenchymal Stem Cells via Inhibiting Hedgehog Signaling Pathway in Preeclampsia.

Authors - Yanqi Zhong
View Article
mouseWB

Phytother Res

Total flavonoid extract from Dracocephalum moldavica L. improves pulmonary fibrosis by reducing inflammation and inhibiting the hedgehog signaling pathway

Authors - Cheng-Hui He
View Article
humanWB

Int J Oncol

Effects of three-dimensional collagen scaffolds on the expression profiles and biological functions of glioma cells.

Authors - Wei Jia
View Article