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- KD/KO Validated
GOLGA2/GM130 Polyclonal antibody
GOLGA2/GM130 Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, Canine and More (2)
Applications
FC, IF, IHC, WB, ELISA
Conjugate
Unconjugated
105
验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, HeLa cells, human spleen tissue, HEK-293 cells, MCF-7 cells |
Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells, HEK-293 cells, HepG2 cells, MDCK cells |
Positive FC detected in | HEK-293 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:200 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
11308-1-AP targets GOLGA2/GM130 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, Canine samples.
Tested Applications | FC, IF, IHC, WB, ELISA |
Cited Applications | IF, IHC, WB |
Tested Reactivity | human, Canine |
Cited Reactivity | human, monkey, Hamster |
Immunogen | GOLGA2/GM130 fusion protein Ag1848 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | golgi autoantigen, golgin subfamily a, 2 |
Synonyms | Cell and organelle markers, GM130, GM130 autoantigen, GOLGA2, Golgi Marker, Golgin 95, Golgin subfamily A member 2 |
Calculated Molecular Weight | 111 kDa |
Observed Molecular Weight | 130 kDa |
GenBank Accession Number | BC014188 |
Gene Symbol | GOLGA2 |
Gene ID (NCBI) | 2801 |
RRID | AB_2115327 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q08379 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for GOLGA2/GM130 antibody 11308-1-AP | Download protocol |
IHC protocol for GOLGA2/GM130 antibody 11308-1-AP | Download protocol |
IF protocol for GOLGA2/GM130 antibody 11308-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Immunity The nucleotide receptor STING translocates to the phagosomes to negatively regulate anti-fungal immunity | ||
Nat Methods Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM). | ||
Cell Metab Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans. | ||
Cell Metab TMEM41B acts as an ER scramblase required for lipoprotein biogenesis and lipid homeostasis. | ||
Nat Cell Biol Manganese regulation of COPII condensation controls circulating lipid homeostasis |