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Ribosomal protein L4 Polyclonal antibody

Ribosomal protein L4 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

FC, IF, IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 11302-1-AP

Print datasheet

Synonyms

60S ribosomal protein L1, 60S ribosomal protein L4, ribosomal protein L4, RPL1, RPL4



经过测试的应用

Positive WB detected inHeLa cells, rat skeletal muscle tissue, HepG2 cells, MCF-7 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11302-1-AP targets Ribosomal protein L4 in WB, RIP, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF, IHC, IP, WB, ELISA
Cited Applications FC, IF, IP, RIP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, Arabidopsis, rice
Immunogen Ribosomal protein L4 fusion protein Ag1828 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribosomal protein L4
Synonyms 60S ribosomal protein L1, 60S ribosomal protein L4, ribosomal protein L4, RPL1, RPL4
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC009888
Gene Symbol RPL4
Gene ID (NCBI) 6124
RRIDAB_2181909
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP36578
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

60S ribosomal protein L4 is encoded by RPL4 (or RPL1) gene. RPL4 is a component of 60S subunit of ribosome, and belongs to the L4E family. By interacting with c-Myb, RPL4 plays a important role in c-myc expression in response to growth factor and nutritional signals. Recently RPL4 was reported to be able to increase effeiciency of viral recoding sequence.

实验方案

Product Specific Protocols
WB protocol for Ribosomal protein L4 antibody 11302-1-APDownload protocol
IHC protocol for Ribosomal protein L4 antibody 11302-1-APDownload protocol
IF protocol for Ribosomal protein L4 antibody 11302-1-APDownload protocol
IP protocol for Ribosomal protein L4 antibody 11302-1-APDownload protocol
FC protocol for Ribosomal protein L4 antibody 11302-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Science

The Ccr4-Not complex monitors the translating ribosome for codon optimality.

Authors - Robert Buschauer
humanWB

Cell

Ribosome Collisions Trigger General Stress Responses to Regulate Cell Fate.

Authors - Colin Chih-Chien Wu
mouseIF

Cell Res

Pre-ribosomal RNA reorganizes DNA damage repair factors in nucleus during meiotic prophase and DNA damage response.

Authors - Xiaochen Gai
humanWB

Mol Cell

N6-Methyladenosine Guides mRNA Alternative Translation during Integrated Stress Response.

Authors - Jun Zhou
humanWB

Mol Cell

The G3BP1-Family-USP10 Deubiquitinase Complex Rescues Ubiquitinated 40S Subunits of Ribosomes Stalled in Translation from Lysosomal Degradation.

Authors - Cindy Meyer
humanWB, IP

Nat Struct Mol Biol

Translational control of the cytosolic stress response by mitochondrial ribosomal protein L18.

Authors - Xingqian Zhang