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Ribosomal protein L4 Polyclonal antibody
Ribosomal protein L4 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
FC, IF, IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, rat skeletal muscle tissue, HepG2 cells, MCF-7 cells |
Positive IP detected in | K-562 cells |
Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Positive FC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
11302-1-AP targets Ribosomal protein L4 in WB, RIP, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | FC, IF, IHC, IP, WB, ELISA |
Cited Applications | FC, IF, IP, RIP, WB |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, Arabidopsis, rice |
Immunogen | Ribosomal protein L4 fusion protein Ag1828 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | ribosomal protein L4 |
Synonyms | 60S ribosomal protein L1, 60S ribosomal protein L4, ribosomal protein L4, RPL1, RPL4 |
Calculated Molecular Weight | 48 kDa |
Observed Molecular Weight | 48 kDa |
GenBank Accession Number | BC009888 |
Gene Symbol | RPL4 |
Gene ID (NCBI) | 6124 |
RRID | AB_2181909 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P36578 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Ribosomal protein L4 antibody 11302-1-AP | Download protocol |
IHC protocol for Ribosomal protein L4 antibody 11302-1-AP | Download protocol |
IF protocol for Ribosomal protein L4 antibody 11302-1-AP | Download protocol |
IP protocol for Ribosomal protein L4 antibody 11302-1-AP | Download protocol |
FC protocol for Ribosomal protein L4 antibody 11302-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Res Pre-ribosomal RNA reorganizes DNA damage repair factors in nucleus during meiotic prophase and DNA damage response. | ||
Mol Cell N6-Methyladenosine Guides mRNA Alternative Translation during Integrated Stress Response. | ||
Mol Cell The G3BP1-Family-USP10 Deubiquitinase Complex Rescues Ubiquitinated 40S Subunits of Ribosomes Stalled in Translation from Lysosomal Degradation. | ||
Nat Struct Mol Biol Translational control of the cytosolic stress response by mitochondrial ribosomal protein L18. |