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EIF2S1 Polyclonal antibody

EIF2S1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

FC, IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 11170-1-AP

Print datasheet

Synonyms

EIF 2, eIF 2 alpha, EIF 2A, EIF 2alpha, EIF2, EIF2A, EIF2S1/EIF2A, eIF2α



经过测试的应用

Positive WB detected inHepG2 cells, Jurkat cells, MCF-7 cells, NIH/3T3 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite® Plus 555 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11170-1-AP targets EIF2S1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF, IHC, IP, WB, ELISA
Cited ApplicationsIHC, IP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Immunogen EIF2S1 fusion protein Ag1645 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
Synonyms EIF 2, eIF 2 alpha, EIF 2A, EIF 2alpha, EIF2, EIF2A, EIF2S1/EIF2A, eIF2α
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC002513
Gene Symbol EIF2S1
Gene ID (NCBI) 1965
RRIDAB_2096489
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP05198
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EIF2S1 is one subunit of the translation initiation factor EIF2, which catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. EIF2A (Gene ID: 83939) and EIF2S1 (Gene ID: 1965) share the EIF2A symbol/alias in common. EIF2S1 is the alpha subunit of the eIF2 translation initiation complex. Although both of these proteins function in binding initiator tRNA to the 40S ribosomal subunit, the EIF2A protein does so in a codon-dependent manner, whereas eIF2 complex requires GTP.

实验方案

Product Specific Protocols
WB protocol for EIF2S1 antibody 11170-1-APDownload protocol
IHC protocol for EIF2S1 antibody 11170-1-APDownload protocol
IF protocol for EIF2S1 antibody 11170-1-APDownload protocol
IP protocol for EIF2S1 antibody 11170-1-APDownload protocol
FC protocol for EIF2S1 antibody 11170-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

Mitochondrial DNA breaks activate an integrated stress response to reestablish homeostasis

Authors - Yi Fu
humanWB

J Immunother Cancer

Loss of ADAR1 in macrophages in combination with interferon gamma suppresses tumor growth by remodeling the tumor microenvironment

Authors - Weiwei Lin
humanWB

Mol Cancer

Exosomal circSHKBP1 promotes gastric cancer progression via regulating the miR-582-3p/HUR/VEGF axis and suppressing HSP90 degradation.

Authors - Mengyan Xie
humanWB

Redox Biol

Mecheliolide elicits ROS-mediated ERS driven immunogenic cell death in hepatocellular carcinoma.

Authors - Zhongren Xu
pigWB

Autophagy

Inhibition of USP14 influences alphaherpesvirus proliferation by degrading viral VP16 protein via ER stress-triggered selective autophagy.

Authors - Sheng-Li Ming
pigWB

Autophagy

STING1 is essential for an RNA-virus triggered autophagy.

Authors - Rui Zhang