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GADD34 Polyclonal antibody

GADD34 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (4)

Applications

IF, IHC, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 10449-1-AP

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Synonyms

GADD34, growth arrest and DNA damage-inducible protein 34, PPP1R15A



经过测试的应用

Positive WB detected inMG132 treated NIH/3T3 cells, Tunicamycin treated HeLa cells, PC-3 cells, K-562 cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman pancreas tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10449-1-AP targets GADD34 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF, IHC, WB, ELISA
Cited Applications FC, IF, IHC, IP, WB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, monkey, hamster, pig
Immunogen GADD34 fusion protein Ag0578 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein phosphatase 1, regulatory (inhibitor) subunit 15A
Synonyms GADD34, growth arrest and DNA damage-inducible protein 34, PPP1R15A
Calculated Molecular Weight 73 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC003067
Gene Symbol PPP1R15A
Gene ID (NCBI) 23645
RRIDAB_2168724
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75807
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GADD34, also named PPP1R15A, belongs to the PPP1R15 family. GADD34 can be triggered as a direct target of activating transcription factor4 (ATF4) under ER stress, it plays a pivotal role in the recovery of cells from shut-down of translation induced by ER stress. It recruits the serine/threonine-protein phosphatase (PP1) to dephosphorylate the translation initiation factor eIF2alpha, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. GADD34 down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 via PP1. It may also promote apoptosis by inducing TP53 phosphorylation on 'Ser-15'. Starvation-induced expression of GADD34 reduced mTOR activity and induced autophagy in wild-type mice, but not in GADD34 KO mice. Molecular weight of GADD34 is 100 kDa confirmed in GADD34 KO mice, and Proteintech's GADD34 antibody 10449-1-AP primarily recongize the 100 kDa band.

实验方案

Product Specific Protocols
WB protocol for GADD34 antibody 10449-1-APDownload protocol
IHC protocol for GADD34 antibody 10449-1-APDownload protocol
IF protocol for GADD34 antibody 10449-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

An inhibitor of NEDD8-activating enzyme as a new approach to treat cancer.

Authors - Soucy Teresa A TA
mouseWB,IHC

Nature

Sustained translational repression by eIF2α-P mediates prion neurodegeneration.

Authors - Moreno Julie A JA
humanWB

Science

Preventing proteostasis diseases by selective inhibition of a phosphatase regulatory subunit.

Authors - Indrajit Das
humanWB

Cell

Target-Based Discovery of an Inhibitor of the Regulatory Phosphatase PPP1R15B.

Authors - Agnieszka Krzyzosiak
humanWB, IP

Science

Selective inhibition of a regulatory subunit of protein phosphatase 1 restores proteostasis.

Authors - Pavel Tsaytler
humanWB

Nat Med

A small-molecule inhibitor of the ubiquitin activating enzyme for cancer treatment.

Authors - Marc L Hyer