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ZO-1 Polyclonal antibody

ZO-1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, canine, mouse, rat, hamster and More (5)

Applications

FC, IF, IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 21773-1-AP

Print datasheet

Synonyms

Tight junction protein 1, Tight junction protein ZO 1, TJP1, ZO 1, ZO1, ZO-1, Zona occludens protein 1, Zonula occludens protein 1



经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, COLO 320 cells, HT-29 cells, HUVEC cells, HepG2 cells, mouse brain tissue, rat brain tissue, MCF-7 cells, Caco-2 cells, HeLa cells, K-562 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells, HUVEC cells, MDCK cells
Positive FC detected inMCF-7 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:1000-1:4000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

产品信息

21773-1-AP targets ZO-1 in WB, RIP, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, canine, mouse, rat, hamster samples.

Tested Applications FC, IF, IHC, IP, WB, ELISA
Cited Applications ELISA, FC, IF, RIP, WB
Tested Reactivity human, canine, mouse, rat, hamster
Cited Reactivityhuman, mouse, rat, goat, chicken, rabbit, pig, canine, bovine
Immunogen ZO-1 fusion protein Ag16454 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tight junction protein 1 (zona occludens 1)
Synonyms Tight junction protein 1, Tight junction protein ZO 1, TJP1, ZO 1, ZO1, ZO-1, Zona occludens protein 1, Zonula occludens protein 1
Calculated molecular weight 1748 aa, 195 kDa
Observed molecular weight 230 kDa
GenBank accession numberBC111712
Gene symbol TJP1
UniprotQ07157
Gene ID (NCBI) 7082
RRIDAB_10733242
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

What is the function of ZO-1?

Zona Occludens 1 (ZO-1) is a tight junction (TJ) protein found in complexes at cell-cell contacts. The role of ZO-1 is to recruit other TJ proteins.1,2 The resulting TJ complexes regulate paracellular flow, contribute to apical-basal polarity, and are part of signaling pathways for proliferation and differentiation.3 This protein is useful for highlighting cell-cell contacts both in cultured cells and in tissue samples, outlining cell membranes and specific structures.

 

Where is ZO-1 expressed?

The protein is localized to the cytoplasmic membrane in most types of cells, particularly where a barrier function is essential. It is common in endothelial cells,4 which line the internal surface of blood and lymph vessels, and in epithelial cells,5 which form the outer barrier of organs.

ZO-1 is expressed in many tissues including the intestine, kidney, liver, and skeletal muscle cells.

 

What proteins does ZO-1 interact with?

The molecular weight of ZO-1 is 220kDa6 and it contains multiple distinct protein domains that allow bind to other junctional proteins at the cytoplasmic membrane. The N-terminal of ZO-1 can dimerize with other ZO proteins and bind directly to other TJ proteins including claudins, connexions, and JAMs,7-10 which allows it to assemble TJ complexes. The C-terminal can interact with actin and cortactin, anchoring the TJ complexes to the cytoskeleton.8,11 This suggests that ZO-1 forms a link between the outer cell-cell contacts and the inner actin.

 

1.     McNeil, E., Capaldo, C. T. & Macara, I. G. Zonula occludens-1 function in the assembly of tight junctions in Madin-Darby canine kidney epithelial cells. Mol. Biol. Cell 17, 1922-32 (2006).

2.     Kratzer, I. et al. Complexity and developmental changes in the expression pattern of claudins at the blood-CSF barrier. Histochem. Cell Biol. (2012). doi:10.1007/s00418-012-1001-9

3.     Guillemot, L., Paschoud, S., Pulimeno, P., Foglia, A. & Citi, S. The cytoplasmic plaque of tight junctions: A scaffolding and signalling center. Biochim. Biophys. Acta - Biomembr. 1778, 601-613 (2008).

4.     Tornavaca, O. et al. ZO-1 controls endothelial adherens junctions, cell-cell tension, angiogenesis, and barrier formation. J. Cell Biol. 208, 821-38 (2015).

5.     Umeda, K. et al. Establishment and characterization of cultured epithelial cells lacking expression of ZO-1. J. Biol. Chem. 279, 44785-94 (2004).

6.     Stevenson, B. R. Identification of ZO-1: a high molecular weight polypeptide associated with the tight junction (zonula occludens) in a variety of epithelia. J. Cell Biol. 103, 755-766 (1986).

7.     Itoh, M. et al. Direct Binding of Three Tight Junction-Associated Maguks, Zo-1, Zo-2, and Zo-3, with the Cooh Termini of Claudins. J. Cell Biol. 147, 1351-1363 (1999).

8.     Fanning, A. S., Jameson, B. J., Jesaitis, L. A. & Anderson, J. M. The Tight Junction Protein ZO-1 Establishes a Link between the Transmembrane Protein Occludin and the Actin Cytoskeleton. J. Biol. Chem. 273, 29745-29753 (1998).

9.     Kausalya, P. J., Reichert, M. & Hunziker, W. Connexin45 directly binds to ZO-1 and localizes to the tight junction region in epithelial MDCK cells. FEBS Lett. 505, 92-96 (2001).

10. Itoh, M. et al. Junctional adhesion molecule (JAM) binds to PAR-3. J. Cell Biol. 154, 491-498 (2001).

11. Itoh, M., Nagafuchi, A., Moroi, S. & Tsukita, S. Involvement of ZO-1 in Cadherin-based Cell Adhesion through Its Direct Binding to α Catenin and Actin Filaments. J. Cell Biol. 138, 181-192 (1997).  

实验方案

Product Specific Protocols
WB protocol for ZO-1 antibody 21773-1-APDownload protocol
IHC protocol for ZO-1 antibody 21773-1-APDownload protocol
IF protocol for ZO-1 antibody 21773-1-APDownload protocol
IP protocol for ZO-1 antibody 21773-1-APDownload protocol
FC protocol for ZO-1 antibody 21773-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse

Am J Respir Crit Care Med

Ezrin, a Membrane Cytoskeleton Cross Linker Protein, as a Marker of Epithelial Damage in Asthma.

Authors - Man Jia
human,mouseIF

Cell Metab

Gut microbiota modulate distal symmetric polyneuropathy in patients with diabetes

Authors - Junpeng Yang
humanIF

Cell Stem Cell

Human branching cholangiocyte organoids recapitulate functional bile duct formation.

Authors - Floris J M Roos
IF

Microbiome

Arula-7 powder improves diarrhea and intestinal epithelial tight junction function associated with its regulation of intestinal flora in calves infected with pathogenic Escherichia coli O1

Authors - Hao Chen
mouseIF

Adv Sci (Weinh)

Plasma-Activated Solutions Mitigates DSS-Induced Colitis via Restoring Redox Homeostasis and Reversing Microbiota Dysbiosis

Authors - Tuanhe Sun
humanWB

J Extracell Vesicles

Extracellular vesicle-mediated delivery of circDYM alleviates CUS-induced depressive-like behaviours.

Authors - Xiaoyu Yu