Proteintech offers pre-made western blot membranes. Proteins from various human
(normal & diseased), mouse, and rat tissues and cell lines were subjected to SDS PAGE
before being transferred onto PVDF membranes. These membranes are ready to be used
with antibodies. These products can streamline the procedures involved in tissue
collection and lysate preparation. Custom pre-made western blot membranes can be
ordered if you are unable to find the combination you require. (CAT# M90000) These
products must be stored at -20°C or 4°C in sealed bags.
Custom Pre-Made Blot Service: Select any of
the 9 kinds of samples from our lysate list to make your own combination.
List of Pre-made Western Blot:
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Human Cell Line Blot (1)
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Catalog No. : M10001
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Lane 1: HEK293 cell
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Lane 6: Human chronic myelogenous leukemia K562 cell
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Lane 2: Hela cell
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Lane 7: Human melanoma A375
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Lane 3: Human neuroblastoma SH-SY5Y cell
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Lane 8: Human Retinoblastoma Y79
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Lane 4: Human Burkitt's lymphoma Raji Cell
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Lane 9: Human epidermoid carcinoma A549
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Lane 5: Human histiocytic lymphoma U937
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Lane 10: Prestained Protein Marker (see
details below)
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Human Cell Line Blot (2)
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Catalog No. : M10002
|
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Lane 1: HEK293 cell
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Lane 6: Human pancreatic adenocarcinoma BXPC-3 cell
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Lane 2: Hela cell
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Lane 7: Human prostate cancer PC-3 cell
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Lane 3: Human breast adenocarcinoma MCF-7 cell
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Lane 8: A Human lung carcinoma A549 cell
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Lane 4: Human breast Carcinoma MDA-MB-453 cell
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Lane 9: Human gastric cancer SGC-7901 cell
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Lane 5: Human hepatocellular liver carcinoma HEPG2 cell
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Lane 10: Prestained Protein Marker (see
details below)
|
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Human Tissue Blot- Fetus Normal (1)
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Catalog No. : M10003
|
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Lane 1: Human brain(frontal lobe)
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Lane 6: Human heart(left auricela)
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Lane 2: Human brain(occipital lobe)
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Lane 7: Human interventricular septum)
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Lane3: Human brain(occipital lobe)
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Lane 8: Human heart(right atrium)
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Lane 4: Human cerebellum
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Lane 9: Human heart(right auricela)
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Lane 5: Human heart(left atrium)
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Lane 10: Prestained Protein Marker (see
details below)
|
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Human Tissue Blot- Fetus Normal (2)
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Catalog No. : M10004
|
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Lane 1: Human brain
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Lane 6: Human lymph node
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Lane 2: Human colon
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Lane 7: Human pancreas
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Lane 3: Human heart
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Lane 8: Human skin
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Lane 4: Human kidney
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Lane 9: Human testis
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Lane 5: Human liver
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Lane 10: Prestained Protein Marker (see
details below)
|
|
Mouse Tissue Blot
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Catalog No. : M10005
|
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Lane 1: Mouse brain
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Lane 6: Mouse lymph node
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Lane 2: Mouse colon
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Lane 7: Mouse pancreas
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Lane 3: Mouse heart
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Lane 8: Mouse skin
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Lane 4: Mouse kidney
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Lane 9: Mouse testis
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Lane 5: Mouse liver
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Lane 10: Prestained Protein Marker (see
details below)
|
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Rat Tissue Blot
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Catalog No. : M10006
|
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Lane 1: Rat brain
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Lane 6: Rat lymph node
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Lane 2: Rat colon
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Lane 7: Rat pancreas
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Lane 3: Rat heart
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Lane 8: Rat skin
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Lane 4: Rat kidney
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Lane 9: Rat testis
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Lane 5: Rat liver
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Lane 10: Prestained Protein Marker (see
details below)
|
Pre-stained protein marker consists of 6 proteins :
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Marker#
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Protein
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Marker Color
|
Mol Wt (kDa)
|
|
1
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a-Lactalbumin
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Yellow
|
14
|
|
2
|
Trypsin Inhibitor
|
Blue
|
20
|
|
3
|
Carbonic Anhydrase
|
Orange
|
30
|
|
4
|
Egg Albumin
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Red
|
40
|
|
5
|
BSA
|
Cyan
|
62
|
|
6
|
Phosphorylase b
|
Grey
|
94
|
