CoraLite®594-conjugated Vimentin Monoclonal antibody
Vimentin Monoclonal Antibody for FC (Intra), IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
FC (Intra), IF/ICC
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
3H9D1
验证数据展示
经过测试的应用
| Positive IF detected in | HepG2 cells |
| Positive FC detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 60330-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 2 publications below |
产品信息
CL594-60330 targets Vimentin in IF, FC(Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | FC (Intra), IF/ICC |
| Cited Applications | IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Immunogen | Vimentin fusion protein Ag0489 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | vimentin |
| Synonyms | Cell and organelle markers, Cytoskeleton Marker, FLJ36605, VIM, vimentin |
| Calculated Molecular Weight | 466 aa, 54 kDa |
| GenBank Accession Number | BC000163 |
| Gene Symbol | VIM |
| Gene ID (NCBI) | 7431 |
| ENSEMBL Gene ID | ENSG00000026025 |
| RRID | AB_2883466 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P08670 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. The antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 Vimentin antibody CL594-60330 | Download protocol |
| FC protocol for CL594 Vimentin antibody CL594-60330 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mater Today Bio Engineered muscle from micro-channeled PEG scaffold with magnetic Fe3O4 fixation towards accelerating esophageal muscle repair | ||
Front Cell Dev Biol Ectopic Endometrial Cell-Derived Exosomal Moesin Induces Eutopic Endometrial Cell Migration, Enhances Angiogenesis and Cytosolic Inflammation in Lesions Contributes to Endometriosis Progression. |
