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  • KD/KO Validated

TROAP Polyclonal antibody

TROAP Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 13634-1-AP

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Synonyms

TASTIN, TROAP, Trophinin assisting protein, Trophinin associated protein



经过测试的应用

Positive WB detected inHeLa cells, HCT 116 cells, PC-3 cells, HT-29 cells, Jurkat cells
Positive IHC detected inhuman gliomas tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13634-1-AP targets TROAP in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB, ELISA
Cited Applications IHC, WB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen TROAP fusion protein Ag4562 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name trophinin associated protein (tastin)
Synonyms TASTIN, TROAP, Trophinin assisting protein, Trophinin associated protein
Calculated Molecular Weight 778 aa, 84 kDa
Observed Molecular Weight110 kDa
GenBank Accession NumberBC032583
Gene Symbol TROAP
Gene ID (NCBI) 10024
RRIDAB_10597857
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12815
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TROAP, also known as tastin, was first cloned from human epithelial cells in 1995. It as a cytoplasmic protein is composed of 778 amino acid residues and contains potential phosphorylation sites for protein kinases. TROAP highly expresses in bone marrow, testis and thymus, and is involved in centrosome integrity and spindle assembly during mitosis (PMID: 33500384). TROAP has been shown to promote some tumor progressions, such as those of breast and colorectal cancers (PMID: 33692939).

实验方案

Product Specific Protocols
WB protocol for TROAP antibody 13634-1-APDownload protocol
IHC protocol for TROAP antibody 13634-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Dev Cell

The HCMV Assembly Compartment Is a Dynamic Golgi-Derived MTOC that Controls Nuclear Rotation and Virus Spread.

Authors - Dean J Procter
humanIHC

Brief Bioinform

Comprehensive assessment of cellular senescence in the tumor microenvironment.

Authors - Xiaoman Wang
humanWB

Biomed Pharmacother

MicroRNA-519d-3p inhibits cell proliferation and migration by targeting TROAP in colorectal cancer.

Authors - Xiaoyong Ye
  • KD Validated
humanWB

Cell Cycle

MiR-532-3p suppresses cell viability, migration and invasion of clear cell renal cell carcinoma through targeting TROAP.

Authors - Bin Gao
humanWB

Front Oncol

Downregulation of Methyltransferase-Like 14 Promotes Ovarian Cancer Cell Proliferation Through Stabilizing TROAP mRNA.

Authors - Yize Li
humanWB

CNS Neurosci Ther

TROAP regulates cell cycle and promotes tumor progression through Wnt/β-Catenin signaling pathway in glioma cells.

Authors - Zong-Qing Zhao