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  • KD/KO Validated

TCHP Polyclonal antibody

TCHP Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 25931-1-AP

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Synonyms

Protein TCHP, TCHP, Tumor suppressor protein



经过测试的应用

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman liver tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25931-1-AP is TCHP Fusion Protein expressed in E. coli.

Tested Applications IF, IHC, WB,ELISA
Cited Applications IF, WB
Tested Reactivity human
Cited Reactivityhuman
Immunogen TCHP fusion protein Ag23199 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name trichoplein, keratin filament binding
Synonyms Protein TCHP, TCHP, Tumor suppressor protein
Calculated Molecular Weight 61 kDa
Observed Molecular Weight 61 kDa
GenBank Accession NumberBC004285
Gene Symbol TCHP
Gene ID (NCBI) 84260
RRIDAB_2880300
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BT92
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TCHP antibody 25931-1-APDownload protocol
IHC protocol for TCHP antibody 25931-1-APDownload protocol
IF protocol for TCHP antibody 25931-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Cell Mol Life Sci

Requirement of NPHP5 in the hierarchical assembly of basal feet associated with basal bodies of primary cilia.

Authors - Delowar Hossain
  • KD Validated
humanWB

Int J Mol Sci

Primary Cilia Restrain PI3K-AKT Signaling to Orchestrate Human Decidualization

Authors - Bo Li
  • KD Validated
humanWB

J Cell Physiol

Cell cycle arrest induced by trichoplein depletion is independent of cilia assembly.

Authors - Min Huang
  • KD Validated