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SIRT1 Monoclonal antibody
SIRT1 Monoclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human and More (1)
Applications
IF/ICC, IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
7F6G6
验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, HeLa cells, Hek-293 cells, LNCaP cells, Jurkat cells, MCF-7 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human breast cancer tissue, human testis tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
60303-1-Ig targets SIRT1 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human samples.
Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
Cited Applications | WB, IF, IHC, CoIP, ELISA |
Tested Reactivity | human |
Cited Reactivity | human, pig |
Immunogen | SIRT1 fusion protein Ag17677 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) |
Synonyms | hSIR2, hSIRT1, SIR2 like protein 1, SIR2L1, SIRT1 |
Calculated Molecular Weight | 747 aa, 82 kDa |
Observed Molecular Weight | 110-130 kDa |
GenBank Accession Number | BC012499 |
Gene Symbol | SIRT1 |
Gene ID (NCBI) | 23411 |
RRID | AB_2881417 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q96EB6 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for SIRT1 antibody 60303-1-Ig | Download protocol |
IHC protocol for SIRT1 antibody 60303-1-Ig | Download protocol |
IF protocol for SIRT1 antibody 60303-1-Ig | Download protocol |
IP protocol for SIRT1 antibody 60303-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Pineal Res Melatonin Protects Mouse Testes from Palmitic Acid-Induced Lipotoxicity by Attenuating Oxidative Stress and DNA Damage in a SIRT1-Dependent Manner. | ||
Free Radic Biol Med Menaquinone-4 prevents medication-related osteonecrosis of the jaw through the SIRT1 signaling-mediated inhibition of cellular metabolic stresses-induced osteoblast apoptosis | ||
Int J Mol Sci Exposure to Bisphenol A Caused Hepatoxicity and Intestinal Flora Disorder in Rats. | ||
Int J Mol Sci COX-2/sEH Dual Inhibitor Alleviates Hepatocyte Senescence in NAFLD Mice by Restoring Autophagy through Sirt1/PI3K/AKT/mTOR. | ||
J Cell Physiol Y-box binding protein 1 influences zygotic genome activation by regulating N6-methyladenosine in porcine embryos | ||
J Cell Physiol Acetyl-CoA synthases are essential for maintaining histone acetylation under metabolic stress during zygotic genome activation in pigs. |