14-3-3 Sigma Monoclonal antibody
14-3-3 Sigma Monoclonal Antibody for FC, IF/ICC, IHC, WB,ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human and More (1)
Applications
FC, IF/ICC, IHC, WB,ELISA
Conjugate
Unconjugated
CloneNo.
3F10D9
验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, HeLa cells, MCF-7 cells |
Positive IHC detected in | human skin tissue, human breast cancer tissue, human breast hyperplasia tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | MCF-7 cells |
Positive FC detected in | A431 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:14000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:8000 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66251-1-Ig targets 14-3-3 Sigma in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
Tested Applications | FC, IF/ICC, IHC, WB,ELISA |
Cited Applications | IF, IHC, WB |
Tested Reactivity | human |
Cited Reactivity | human, mouse |
Immunogen | 14-3-3 Sigma fusion protein Ag12644 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | stratifin |
Synonyms | 14 3 3 protein sigma, HME1, SFN, stratifin, YWHAS |
Calculated Molecular Weight | 28 kDa |
Observed Molecular Weight | 28-30 kDa |
GenBank Accession Number | BC002995 |
Gene Symbol | SFN |
Gene ID (NCBI) | 2810 |
RRID | AB_2881639 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P31947 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for 14-3-3 Sigma antibody 66251-1-Ig | Download protocol |
IHC protocol for 14-3-3 Sigma antibody 66251-1-Ig | Download protocol |
IF protocol for 14-3-3 Sigma antibody 66251-1-Ig | Download protocol |
FC protocol for 14-3-3 Sigma antibody 66251-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Cell Biol Persistence of a regeneration-associated, transitional alveolar epithelial cell state in pulmonary fibrosis. | ||
Mol Biotechnol Stratifin (SFN) Regulates Cervical Cancer Cell Proliferation, Apoptosis, and Cytoskeletal Remodeling and Metastasis Progression Through LIMK2/Cofilin Signaling | ||
Transl Androl Urol Discovery of bladder cancer biomarkers in paired pre- and postoperative urine samples. | ||
Environ Toxicol Multidimensional progressive single-cell sequencing reveals cell microenvironment composition and cancer heterogeneity in lung cancer |