14-3-3 Sigma Polyclonal antibody

14-3-3 Sigma Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 10622-1-AP

Print datasheet

Synonyms

14 3 3 protein sigma, 14-3-3, 14-3-3 sigma, HME1, SFN, stratifin, YWHAS



经过测试的应用

Positive WB detected inA431 cells, A549 cells, HeLa cells, U2OS cells
Positive IHC detected inmouse skin tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy SFN Ready-To-Use IHC Kit. SFN primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

10622-1-AP targets 14-3-3 Sigma in WB, IHC, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IHC, WB, ELISA
Cited Applications WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen 14-3-3 Sigma fusion protein Ag0973 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name stratifin
Synonyms 14 3 3 protein sigma, 14-3-3, 14-3-3 sigma, HME1, SFN, stratifin, YWHAS
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 28-30 kDa
GenBank Accession NumberBC002995
Gene Symbol SFN
Gene ID (NCBI) 2810
RRIDAB_2254479
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP31947
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

14-3-3 Sigma is a member 14-3-3 family of highly conserved proteins participating in diverse cellular processes including cell cycle progression. It is exclusively present in various epithelial cells, and alternatively named as human epithelial marker (HEM), or stratifin. Alteration of its expression has been linked to cancer.

实验方案

Product Specific Protocols
WB protocol for 14-3-3 Sigma antibody 10622-1-APDownload protocol
IHC protocol for 14-3-3 Sigma antibody 10622-1-APDownload protocol
FC protocol for 14-3-3 Sigma antibody 10622-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Sci Rep

Sting and p53 DNA repair pathways are compromised in Alzheimer's disease

Authors - Thomas J Nelson
humanWB

Phytomedicine

Acetylation of MOB1 mediates polyphyllin II-reduced lysosome biogenesis in breast cancer by promoting the cytoplasmic retention of the YAP/TFEB coactivator complex

Authors - Yuliang Ren