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PTEN Monoclonal antibody

PTEN Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

5C10B6

Cat No : 60300-1-Ig

Print datasheet

Synonyms

10q23del, BZS, MHAM, MMAC1, phosphatase and tensin homolog, PTEN, PTEN1, TEP1



经过测试的应用

Positive WB detected inDU 145 cells, fetal human brain tissue, HeLa cells, HEK-293 cells, MCF-7 cells, NIH/3T3 cells, HepG2 cells
Positive IHC detected inhuman breast hyperplasia tissue, human breast cancer tissue, human endometrial cancer tissue, human lung cancer tissue, human prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PTEN Ready-To-Use IHC Kit. PTEN primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:1200
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60300-1-Ig targets PTEN in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF, IHC, WB, ELISA
Cited Applications IF, IHC, WB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen PTEN fusion protein Ag17274 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name phosphatase and tensin homolog
Synonyms 10q23del, BZS, MHAM, MMAC1, phosphatase and tensin homolog, PTEN, PTEN1, TEP1
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC005821
Gene Symbol PTEN
Gene ID (NCBI) 5728
RRIDAB_2881415
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP60484
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PTEN (also designated MMAC1), products of tumor suppressor genes, are found deleted in most human gliomas. The PTEN genes are also mutated in many other tumors, such as brain, breast, kidney and prostate cancers. PTEN is a protein tyrosine phosphatase that may terminate the signaling transduction pathways mediated by PI 3-kinase/Akt. PTEN has an apparent molecular weight of 55 kDa and it is located in the cytosol.

实验方案

Product Specific Protocols
WB protocol for PTEN antibody 60300-1-IgDownload protocol
IHC protocol for PTEN antibody 60300-1-IgDownload protocol
IF protocol for PTEN antibody 60300-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

Fumarate inhibits PTEN to promote tumorigenesis and therapeutic resistance of type2 papillary renal cell carcinoma.

Authors - Xin Ge
humanIHC

Hepatology

Long noncoding RNA TSLNC8 is a tumor suppressor that inactivates the interleukin-6/STAT3 signaling pathway.

Authors - Jiwei Zhang
humanWB

Hepatology

Cyclin G1-mediated epithelial-mesenchymal transition via phosphoinositide 3-kinase/Akt signaling facilitates liver cancer progression.

Authors - Wen Wen W
humanIF

Nat Commun

The deubiquitylase OTUD3 stabilizes GRP78 and promotes lung tumorigenesis.

Authors - Tongde Du
mouseIHC

Cancer Res

Targeting USP7 identifies a metastasis-competent state within bone marrow-resident melanoma CTCs.

Authors - Monika Vishnoi
humanWB

J Transl Med

HNRNPA2B1-mediated m6A modification of lncRNA MEG3 facilitates tumorigenesis and metastasis of non-small cell lung cancer by regulating miR-21-5p/PTEN axis

Authors - Ke Li