Mouse IL-5 ELISA Kit

Sensitivity

1.0 pg/mL

Range

7.8-500 pg/mL

Reactivity

Mouse

Cat No : KE10018

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Synonyms

B-cell growth factor II, BCGF-II, Il 5, Il5, IL-5, interleukin 5, Interleukin-5



Product Information

KE10018 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL-5 ELISA kit is to be used to detect and quantify protein levels of endogenous IL-5. The assay recognizes mouse IL-5. An antibody specific for IL-5 has been pre-coated onto the microwells. The IL-5 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IL-5 is added to detect the captured IL-5 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Mouse IL-5 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 1.0 pg/mL
Range 7.8-500 pg/mL
Reactivity Mouse
Tested applications Sandwich ELISA
Gene ID (NCBI) 16191

Recovery

Sample TypeAverageRange
Mouse serum90%76%-116%
Cell culture supernatants94%82%-108%

IntraAssay

Samplenmean ( pg/mL)SDCV%
12010.30.43.9
220108.02.01.9
320556.421.64.7

InterAssay

Samplenmean ( pg/mL)SDCV%
12410.40.54.9
224111.41.81.6
324503.017.43.5

Background Information

Interleukin-5, or IL-5, was originally discovered as a soluble T cell-derived factor, called T cell-replacing factor (TRF), that induced T cell-depleted activated B cells to secrete immunoglobulin. IL-5 is a key hematopoietic cytokine in eosinophil differentiation, maturation, recruitment and activation at sites of allergic inflammation. IL-5 also plays a role in the development, metabolism, and function of basophils. IL-5 exerts its biological activity through the IL-5 receptor (IL-5R), which is composed of at least two chains: an α chain that binds IL-5 with low affinity and a βchain that does not bind IL-5, but together with the IL-5α chain, constitutes the high affinity IL-5 receptor. The β chain is common to the IL-3, IL-5 and Gm-csf receptors and has been shown to signal through the JAK/Stat pathway.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms B-cell growth factor II, BCGF-II, Il 5, Il5, IL-5, interleukin 5, Interleukin-5

Publications

SpeciesSample TypeTitle
mousecell culture supernatants

Int Immunopharmacol

Nicotinamide mononucleotide attenuates airway epithelial barrier dysfunction via inhibiting SIRT3 SUMOylation in asthma

Authors - Jiayuan Liang
mouseserum

Basic Clin Pharmacol Toxicol

SIRT1/IGFBPrP1/TGF β1 axis involved in cucurbitacin B ameliorating concanavalin A-induced mice liver fibrosis.

Authors - Li Yang

J Food Biochem

Lycoperoside H, a steroidal alkaloid saponin in tomato seeds, ameliorates atopic dermatitis-like symptoms in IL-33 transgenic mice.

Authors - Shogo Takeda
mousetissue homogenates

Nutrients

Anti-Colorectal Cancer Effects of Inonotus hispidus (Bull.: Fr.) P. Karst. Spore Powder through Regulation of Gut Microbiota-Mediated JAK/STAT Signaling

Authors - Hongxin Yang
mouseserum

Int Forum Allergy Rhinol

IL-17A facilitates type 2 inflammation in a modified eosinophilic chronic rhinosinusitis mouse model

Authors - Haotian Wu
mousecell culture supernatants

ACS Omega

Exploring the Mechanism of Bergamot Essential Oil against Asthma Based on Network Pharmacology and Experimental Verification

Authors - Siwen Feng