Cytokeratin 19 Polyclonal antibody

Cytokeratin 19 Polyclonal Antibody for FC, IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (4)

Applications

FC, IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 10712-1-AP

Print datasheet

Synonyms

Cell and organelle markers, CK 19, CK19, Cytokeratin 19, Cytoskeleton Marker, K19, K1CS, keratin 19, KRT19



经过测试的应用

Positive WB detected inA431 cells, HepG2 cells, mouse placenta tissue, MCF-7 cells, A549 cells, mouse brain tissue, HT-29 cells
Positive IHC detected inhuman colon tissue, human pancreas tissue, human thyroid cancer tissue, mouse liver tissue, human oesophagus cancer tissue, mouse skin tissue, mouse colon tissue, human lung cancer tissue, human breast cancer tissue, human stomach cancer tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHaCaT cells, HeLa cells, HepG2 cells, Retinal organoids
Positive FC detected inHT-29 cells
Planning an IHC experiment? We recommend our IHCeasy KRT19 Ready-To-Use IHC Kit. KRT19 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:3000-1:30000
Immunohistochemistry (IHC)IHC : 1:3000-1:12000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10712-1-AP targets Cytokeratin 19 in WB,IHC,IF,FC,ELISA applications and shows reactivity with human, mouse samples.

Tested Applications FC, IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB,IHC,IF,FC
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, goat, pig, dog
Immunogen Cytokeratin 19 fusion protein Ag1085 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name keratin 19
Synonyms Cell and organelle markers, CK 19, CK19, Cytokeratin 19, Cytoskeleton Marker, K19, K1CS, keratin 19, KRT19
Calculated Molecular Weight 40 kDa
Observed Molecular Weight 44-50 kDa
GenBank Accession NumberBC007628
Gene Symbol KRT19
Gene ID (NCBI) 3880
RRIDAB_2133325
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP08727
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 19 is a type I cytokeratin. It is a biochemical marker of skin stem cells in vivo and in vitro.

实验方案

Product Specific Protocols
WB protocol for Cytokeratin 19 antibody 10712-1-APDownload protocol
IHC protocol for Cytokeratin 19 antibody 10712-1-APDownload protocol
IF protocol for Cytokeratin 19 antibody 10712-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC,IF

Nat Commun

Interventional hydrogel microsphere vaccine as an immune amplifier for activated antitumour immunity after ablation therapy

Authors - Xiaoyu Liu
humanIHC

Sci Transl Med

PTEN status determines chemosensitivity to proteasome inhibition in cholangiocarcinoma.

Authors - Tian-Yi Jiang
human

Adv Sci (Weinh)

PMID: 31728273

Authors - Qingming Tang
human

Nat Commun

Reversal of pancreatic desmoplasia by re-educating stellate cells with a tumour microenvironment-activated nanosystem.

Authors - Xuexiang Han
humanIHC

J Clin Invest

CCN1 induces hepatic ductular reaction through integrin αvβ5-mediated activation of NF-κB.

Authors - Ki-Hyun Kim

Cell Host Microbe

Zika Virus Targets Different Primary Human Placental Cells, Suggesting Two Routes for Vertical Transmission.

Authors - Takako Tabata