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Kv1.2 Polyclonal antibody

Kv1.2 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 14235-1-AP

Print datasheet

Synonyms

HBK5, HK4, HUKIV, KCNA2, KV1.2, MK2, NGK1, RBK2

验证数据展示

  • WB analysis using 14235-1-AP

    WB analysis using 14235-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14235-1-AP (Kv1.2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 14235-1-AP

    IHC staining of mouse brain using 14235-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14235-1-AP (Kv1.2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 14235-1-AP

    IHC staining of mouse brain using 14235-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14235-1-AP (Kv1.2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of HeLa using 14235-1-AP

    IP experiment of HeLa using 14235-1-AP

    IP result of anti-Kv1.2 (IP:14235-1-AP, 4ug; Detection:14235-1-AP 1:500) with HeLa cells lysate 5200ug.

经过测试的应用

Positive WB detected inA549 cells, multi-cells/tissue, human heart tissue, HeLa cells, HepG2 cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14235-1-AP targets Kv1.2 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen Kv1.2 fusion protein Ag5477 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name potassium voltage-gated channel, shaker-related subfamily, member 2
Synonyms HBK5, HK4, HUKIV, KCNA2, KV1.2, MK2, NGK1, RBK2
Calculated Molecular Weight 499 aa, 57 kDa
Observed Molecular Weight 66-75 kDa
GenBank Accession NumberBC043564
Gene Symbol KCNA2
Gene ID (NCBI) 3737
RRIDAB_2280576
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP16389
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Kv1.2 antibody 14235-1-APDownload protocol
IHC protocol for Kv1.2 antibody 14235-1-APDownload protocol
IP protocol for Kv1.2 antibody 14235-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols