- Featured Product
- KD/KO Validated
IFT57 Polyclonal antibody
IFT57 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, Canine and More (1)
Applications
IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse retina tissue, HEK-293 cells |
Positive IP detected in | HEK-293 cells |
Positive IHC detected in | human prostate cancer tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | MDCK cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
11083-1-AP targets IFT57 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, Canine samples.
Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
Cited Applications | IF, IP, WB |
Tested Reactivity | human, mouse, rat, Canine |
Cited Reactivity | human, mouse, rat, Chicken |
Immunogen | IFT57 fusion protein Ag1566 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | intraflagellar transport 57 homolog (Chlamydomonas) |
Synonyms | DERP8, ESRRBL1, HIP1 interacting protein, HIPPI, IFT57, MHS4R2 |
Calculated Molecular Weight | 49 kDa |
Observed Molecular Weight | 49 kDa |
GenBank Accession Number | BC011899 |
Gene Symbol | IFT57 |
Gene ID (NCBI) | 55081 |
RRID | AB_2121957 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9NWB7 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for IFT57 antibody 11083-1-AP | Download protocol |
IHC protocol for IFT57 antibody 11083-1-AP | Download protocol |
IF protocol for IFT57 antibody 11083-1-AP | Download protocol |
IP protocol for IFT57 antibody 11083-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Cell Biol The Cep63 paralogue Deup1 enables massive de novo centriole biogenesis for vertebrate multiciliogenesis. | ||
Nat Cell Biol Systematic proteomics of the VCP-UBXD adaptor network identifies a role for UBXN10 in regulating ciliogenesis. | ||
Dev Cell The Intraflagellar Transport Protein IFT27 Promotes BBSome Exit from Cilia through the GTPase ARL6/BBS3. | ||
Nat Commun IFT proteins spatially control the geometry of cleavage furrow ingression and lumen positioning. | ||
EMBO J Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling. | ||
Proc Natl Acad Sci U S A Primary cilia signaling mediates intraocular pressure sensation. |