Human NCALD ELISA Kit

Sensitivity

3 pg/mL

Range

31.25-2000 pg/mL

Reactivity

Human

Cat No : KE00028

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Synonyms

NCALD, neurocalcin delta



Product Information

KE00028 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The NCALD ELISA kit is to be used to detect and quantify protein levels of endogenous NCALD. The assay recognizes human NCALD. A polyclonal antibody specific for NCALD has been pre-coated onto the microwells. The NCALD protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for NCALD is added to detect the captured NCALD protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Product name Human NCALD ELISA Kit
Tests 1 X 96 well plate
Sample type Plasma, Serum
Assay type Sandwich
Sensitivity 3 pg/mL
Range 31.25-2000 pg/mL
Reactivity Human
Tested applications Sandwich ELISA
Gene ID (NCBI) 83988

Recovery

Sample TypeAverageRange
Plasma105%96%-125%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120? ug/ml??%
220? ug/ml??%
320? ug/ml??%

InterAssay

Samplenmean ( pg/mL)SDCV%
120? ug/ml??%
220? ug/ml??%
320? ug/ml??%

Background Information

NCALD belongs to the recoverin family. And it is a member of the EF-hand calcium-binding protein superfamily. NCALD may be involved in the calcium-dependent regulation of rhodopsin phosphorylation. It plays an important role in the regulation of the neuronal signal transduction process. NCALD is a likely candidate for conferring susceptibility to diabetic nephropathy.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms NCALD, neurocalcin delta