Human IL-22 ELISA Kit
Cat No : KE00008
Synonyms
Cytokine Zcyto18, IL 21, IL 22, IL D110, IL TIF, IL21, IL22, IL-22, ILTIF, interleukin 22, TIFa, TIFIL 23, zcyto18
Validation Data Gallery
Product Information
KE00008 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL22 ELISA kit is to be used to detect and quantify protein levels of endogenous IL22. The assay recognizes human IL22. An antibody specific for IL22 has been pre-coated onto the microwells. The IL22 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for IL22 is added to detect the captured IL22 protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human IL-22 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 14.2 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 50616 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plamsa | 113% | 108%-121% |
| Cell culture supernatants | 114% | 101%-119% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 2035.3 | 70.9 | 3.5 |
| 2 | 20 | 539.2 | 26.4 | 4.9 |
| 3 | 20 | 54.8 | 3.6 | 6.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 2174.7 | 155.3 | 7.1 |
| 2 | 24 | 577.5 | 46.8 | 8.1 |
| 3 | 24 | 59.7 | 4.7 | 7.9 |
Background Information
Interleukin 22 (IL22) is a member of the human type I interferon family, which includes IL10. IL22 has the potential to interact with IL10 because it binds to the IL-10R2c chain with IL22R1 in its receptor complex. Binding can be blocked by the soluble receptor, IL22 binding protein (IL22BP). Although known as a Th17 cytokine, IL22 is also expressed by a wide range of immune cells, including NK T, γδ T, and NK cells. IL22 can contribute to immune disease through the stimulation of inflammatory responses, S100s and defensins. IL22 also promotes hepatocyte survival in the liver and epithelial cells in the lung and gut similar to IL10.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Cytokine Zcyto18, IL 21, IL 22, IL D110, IL TIF, IL21, IL22, IL-22, ILTIF, interleukin 22, TIFa, TIFIL 23, zcyto18 |
Publications
| Species | Sample Type | Title |
|---|---|---|
J Neuroimmunol Calcitonin gene-related peptide upregulates IL-17A and IL-22 in γδ-T cells through the paracrine effect of langerhans cells on LC/γδ-T co-culture model | ||
Front Immunol E. coli Nissle 1917 ameliorates mitochondrial injury of granulosa cells in polycystic ovary syndrome through promoting gut immune factor IL-22 via gut microbiota and microbial metabolism |