Human HSP70 ELISA Kit

Sensitivity

1.0 pg/mL

Range

31.25-2000 pg/mL

Reactivity

Human

Cat No : KE00059

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Synonyms

Heat shock 70 kDa protein 1/2, heat shock 70kDa protein 1A, HSP70, HSP70 1, HSP70 1/HSP70 2, HSP70 1A, HSP70.1/HSP70.2, HSP70I, HSP72, HSPA1, HSPA1A, HSPA1B



Product Information

KE000059 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The HSP70 ELISA kit is to be used to detect and quantify protein levels of endogenous HSP70. The assay recognizes human HSP70. An antibody specific for HSP70 has been pre-coated onto the microwells. The HSP70 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody specific for HSP70 is added to detect the captured HSP70 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Human HSP70 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants, Urine
Assay type Sandwich
Sensitivity 1.0 pg/mL
Range 31.25-2000 pg/mL
Reactivity Human
Tested applications Sandwich ELISA
Gene ID (NCBI) 3303

Recovery

Sample TypeAverageRange
Urine86%76%-98%
Human plasma104%92%-124%
Cell culture supernatants97%80%-123%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120182.37.74.2
220448.418.24.1
3201,627.2102.46.3

InterAssay

Samplenmean ( pg/mL)SDCV%
124181.911.16.1
224465.423.45.0
3241,639.490.55.5

Background Information

HSP70 is a stress-inducible member of heat-shock protein 70 (HSP70) proteins which are highly conserved chaperons implicated in protein folding, refolding, transport, and targeting. Expression of HSP70 protein can be highly activated by various stressful stimuli. Significant up-regulation of HSP70 has been found in various tumors. Increased serum HSP70 levels are also associated with progression of multiple diseases, including atherosclerosis, heart failure, and diabetes.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms Heat shock 70 kDa protein 1/2, heat shock 70kDa protein 1A, HSP70, HSP70 1, HSP70 1/HSP70 2, HSP70 1A, HSP70.1/HSP70.2, HSP70I, HSP72, HSPA1, HSPA1A, HSPA1B

Publications

SpeciesSample TypeTitle
humancell culture supernatants

J Clin Invest

Lysosomal exocytosis of HSP70 stimulates monocytic BMP6 expression in Sjögren's syndrome.

Authors - Ying-Qian Mo

Transl Lung Cancer Res

Increased plasma levels of damage-associated molecular patterns during systemic anticancer therapy in patients with advanced lung cancer.

Authors - Hiroyuki Inoue
humanserum

Cancer Med

Changes in serum DAMPs and cytokines/chemokines during near-infrared photoimmunotherapy for patients with head and neck cancer

Authors - Hiromasa Ishihara
humanserum

Surg Obes Relat Dis

The jejunum is the key factor in insulin resistance.

Authors - Lidia Castagneto-Gissey
humanserum

Commun Biol

Metabolic surgery improves insulin resistance through the reduction of gut-secreted heat shock proteins.

Authors - Giulia Angelini
humancell culture supernatants

Cancer Sci

Immunostimulatory oncolytic activity of coxsackievirus A11 in human malignant pleural mesothelioma

Authors - Koji Okamura