Human CD13 ELISA Kit

Sensitivity

0.06 ng/mL

Range

0.313-20 ng/mL

Reactivity

Human

Cat No : KE00083

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Synonyms

Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, ANPEP, AP M, AP N, APN, CD13, gp150, hAPN, LAP1, Microsomal aminopeptidase, p150, PEPN



Product Information

KE00083 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CD13 ELISA kit is to be used to detect and quantify protein levels of endogenous CD13. The assay recognizes human CD13. A polyclonal antibody specific for CD13 has been pre-coated onto the microwells. The CD13 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for CD13 is added to detect the captured CD13 protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Product name Human CD13 ELISA Kit
Tests 1 X 96 well plate
Sample type Plasma, Serum, Cell culture supernatant
Assay type Sandwich
Sensitivity 0.06 ng/mL
Range 0.313-20 ng/mL
Reactivity Human
Tested applications Sandwich ELISA
Gene ID (NCBI) 290

Recovery

Sample TypeAverageRange
Plasma91%79%-105%
Cell culture supernatant106%83%-127%

IntraAssay

Samplenmean ( ng/mL)SDCV%
120? ug/ml??%
220? ug/ml??%
320? ug/ml??%

InterAssay

Samplenmean ( ng/mL)SDCV%
120? ug/ml??%
220? ug/ml??%
320? ug/ml??%

Background Information

CD13 is also named as APN, ANPEP(aminopeptidase N), PEPN and belongs to the peptidase M1 family. It is a 150 kDa cell surface glycoprotein originally identified on subsets of normal and malignant human myeloid cells CD13 reduced ROS-induced DNA damage after genotoxic chemo/radiation stress and protected cells from apoptosis. CD13 is identified as a protein marker correlating with an enrichment of dormant, slow-growing malignant stem cells, with most CD13+ cells resting in the G1/G0 phase of the cell cycle.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, ANPEP, AP M, AP N, APN, CD13, gp150, hAPN, LAP1, Microsomal aminopeptidase, p150, PEPN