- Featured Product
- KD/KO Validated
HADHA Monoclonal antibody
HADHA Monoclonal Antibody for IF/ICC, IHC, WB,ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse
Applications
IF/ICC, IHC, WB,ELISA
Conjugate
Unconjugated
CloneNo.
3E9B1
验证数据展示
经过测试的应用
Positive WB detected in | A375 cells, HEK293 cells |
Positive IHC detected in | human heart tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 1 publications below |
WB | See 7 publications below |
产品信息
The immunogen of 60250-1-Ig is HADHA Fusion Protein expressed in E. coli.
Tested Applications | IF/ICC, IHC, WB,ELISA |
Cited Applications | WB |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Immunogen | HADHA fusion protein Ag1211 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit |
Synonyms | 78 kDa gastrin binding protein, ECHA, HADH, HADHA, LCHAD, MTPA, TP ALPHA |
Calculated Molecular Weight | 83 kDa |
Observed Molecular Weight | 79 kDa |
GenBank Accession Number | BC009235 |
Gene Symbol | HADHA |
Gene ID (NCBI) | 3030 |
RRID | AB_2881371 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P40939 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for HADHA antibody 60250-1-Ig | Download protocol |
IHC protocol for HADHA antibody 60250-1-Ig | Download protocol |
IF protocol for HADHA antibody 60250-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Commun Biol A G1528C Hadha knock-in mouse model recapitulates aspects of human clinical phenotypes for long-chain 3-hydroxyacyl-CoA dehydrogenase deficiency | ||
Proteomics Quantitative Proteomic Study of Myocardial Mitochondria in Urea Transporter B Knockout Mice.
| ||
Cell Biosci Exosomes from hyperglycemia-stimulated vascular endothelial cells contain versican that regulate calcification/senescence in vascular smooth muscle cells. | ||
Science Spermidine activates mitochondrial trifunctional protein and improves antitumor immunity in mice | ||
RSC Adv Glycolytic and lipid oxidative metabolic programs are essential for freshly-isolated regulatory T cells in mice with sepsis |