Glutamate receptor 2 Polyclonal antibody
Glutamate receptor 2 Polyclonal Antibody for WB,IP,IHC,IF-P,FC (Intra),ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB,IP,IHC,IF-P,FC (Intra),ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, rat brain tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | mouse eye tissue |
Positive FC detected in | SH-SY5Y cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:12000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
11994-1-AP targets Glutamate receptor 2 in WB, IP, IF, IHC, CoIP, ELISA, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB,IP,IHC,IF-P,FC (Intra),ELISA |
Cited Applications | WB, IP, IF, IHC, CoIP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | Glutamate receptor 2 fusion protein Ag2662 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | glutamate receptor, ionotropic, AMPA 2 |
Synonyms | GluA2, GluR 2, GluR B, GluR K2, GLUR2, GLURB, Glutamate receptor 2, GRIA2, HBGR2 |
Calculated Molecular Weight | 883 aa, 99 kDa |
Observed Molecular Weight | 94-105 kDa |
GenBank Accession Number | BC010574 |
Gene Symbol | GRIA2 |
Gene ID (NCBI) | 2891 |
RRID | AB_2113725 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P42262 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for Glutamate receptor 2 antibody 11994-1-AP | Download protocol |
IHC protocol for Glutamate receptor 2 antibody 11994-1-AP | Download protocol |
IF protocol for Glutamate receptor 2 antibody 11994-1-AP | Download protocol |
IP protocol for Glutamate receptor 2 antibody 11994-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Biotechnol Magnify is a universal molecular anchoring strategy for expansion microscopy | ||
Sci Adv RNF220 is an E3 ubiquitin ligase for AMPA receptors to regulate synaptic transmission | ||
Mol Psychiatry A recurrent SHANK1 mutation implicated in autism spectrum disorder causes autistic-like core behaviors in mice via downregulation of mGluR1-IP3R1-calcium signaling. | ||
J Clin Invest TMEM25 modulates neuronal excitability and NMDA receptor subunit NR2B degradation. | ||
Mol Neurobiol A Regulatory Circuitry Between Gria2, miR-409, and miR-495 Is Affected by ALS FUS Mutation in ESC-Derived Motor Neurons. | ||