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  • KD/KO Validated

ECHS1 Polyclonal antibody

ECHS1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 11305-1-AP

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Synonyms

ECHS1, ECoH-1, Enoyl CoA hydratase 1, SCEH



经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, mouse heart, mouse liver, rat liver
Positive IP detected inPC-3 cells
Positive IHC detected inhuman prostate cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ECHS1 Ready-To-Use IHC Kit. ECHS1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11305-1-AP targets ECHS1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, IHC, IP, WB, ELISA
Cited Applications IF, IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen ECHS1 fusion protein Ag1842 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name enoyl Coenzyme A hydratase, short chain, 1, mitochondrial
Synonyms ECHS1, ECoH-1, Enoyl CoA hydratase 1, SCEH
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 29-31 kDa
GenBank Accession NumberBC008906
Gene Symbol ECHS1
Gene ID (NCBI) 1892
RRIDAB_2262166
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP30084
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Enoyl-coenzyme A hydratase (ECHS1) is a mitochondrial protein which catalyzes the hydration of 2-trans-enoyl-coenzyme A intermediates to L-3-hydroxyacyl-coenzyme A, playing key role in metabolism of fatty acids in mitochondria. ECHS1 is highly expressed in muscle, liver and fibroblasts. Altered expression of ECHS1 has been considered as a characteristic feature of mitochondria dysfunction. (23275097, 23235493)

实验方案

Product Specific Protocols
WB protocol for ECHS1 antibody 11305-1-APDownload protocol
IHC protocol for ECHS1 antibody 11305-1-APDownload protocol
IF protocol for ECHS1 antibody 11305-1-APDownload protocol
IP protocol for ECHS1 antibody 11305-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Res

One-hit effects in cancer: altered proteome of morphologically normal colon crypts in familial adenomatous polyposis.

Authors - Yeung Anthony T AT
ratWB

J Proteome Res

Proteomic analysis of left ventricular remodeling in an experimental model of heart failure.

Authors - Cieniewski-Bernard Caroline C
humanWB

J Proteome Res

Novel Proteomic Biomarker Panel for Prediction of Aggressive Metastatic Hepatocellular Carcinoma Relapse in Surgically Resectable Patients.

Authors - Gek San Tan
humanWB

Oncotarget

Quantitative proteomics reveals molecular mechanism of gamabufotalin and its potential inhibition on Hsp90 in lung cancer.

Authors - Liyuan Zhang
mouseWB

Am J Pathol

The Krebs cycle and mitochondrial mass are early victims of endothelial dysfunction: proteomic approach.

Authors - Addabbo Francesco F
humanWB

J Med Genet

Strategic validation of variants of uncertain significance in ECHS1 genetic testing

Authors - Yoshihito Kishita
  • KO Validated