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CoraLite® Plus 488-conjugated Cytokeratin 18 Monoclonal antibody

Cytokeratin 18 Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, canine

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G11C4

Cat No : CL488-66187

Print datasheet

Synonyms

CK 18, CYK18, Cytokeratin 18, K18, keratin 18, KRT18

验证数据展示

  • IF Staining of HeLa using CL488-66187

    IF Staining of HeLa using CL488-66187

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL488-66187 (Cytokeratin 18 antibody) at dilution of 1:100.

  • FC experiment of HeLa using CL488-66187

    FC experiment of HeLa using CL488-66187

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 488 Anti-Human Cytokeratin 18 (CL488-66187, Clone:1G11C4) (green), and 0.2 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (black). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

经过测试的应用

Positive IF detected inHeLa cells, HepG2 cells, MDCK cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy KRT18 Ready-To-Use IHC Kit. KRT18 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66187-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66187 targets Cytokeratin 18 in IF, FC (Intra) applications and shows reactivity with human, canine samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity human, canine
Immunogen Cytokeratin 18 fusion protein Ag1260 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name keratin 18
Synonyms CK 18, CYK18, Cytokeratin 18, K18, keratin 18, KRT18
Calculated Molecular Weight 48 kDa
GenBank Accession NumberBC000180
Gene Symbol KRT18
Gene ID (NCBI) 3875
RRIDAB_2883278
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP05783
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT18, also named as CYK18, PIG46 and K18, is the most commonly found members of the intermediate filament gene family. KRT18 is a type I keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas. The antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Cytokeratin 18 antibody CL488-66187Download protocol
FC protocol for CL Plus 488 Cytokeratin 18 antibody CL488-66187Download protocol
Standard Protocols
Click here to view our Standard Protocols